Comet assay for assessment of DNA damage in greenhouse workers exposed to pesticides

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dc.authoridCAYIR, AKIN/0000-0002-2014-6635
dc.contributor.authorCayir, Akin
dc.contributor.authorCoskun, Mahmut
dc.contributor.authorCoskun, Munevver
dc.contributor.authorCobanoglu, Hayal
dc.date.accessioned2025-01-27T20:47:52Z
dc.date.available2025-01-27T20:47:52Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: The main goal of the present study was to determine DNA damage in pesticide-exposed greenhouse workers and pesticides non-exposed controls. Materials and methods: The DNA damage was measured by alkaline comet assay method (pH > 13) in 41 greenhouse workers and 45 non-exposed individuals as the control. Pesticide exposure was assessed by duration of working in the greenhouse and pesticide application in the greenhouse time. DNA damage was estimated by arbitrary unit and damage frequency. Results: Arbitrary unit and damage frequency were consistently significantly higher in greenhouse workers than those of the controls (p = 0.001). In terms of gender in greenhouse, DNA damage of female workers was significantly higher than those in male workers (p < 0.05). We found significant correlation between DNA damage and working hours spent. Multiple linear regression analysis showed that working hours in the greenhouse as an indication of pesticide exposure were significantly associated with the DNA damage, which can be attributed to the genotoxic potential of the pesticide mixture. Conclusions: The comet assay is sensitive to detect the damage exposed to chronic effect of pesticides in greenhouse workers. Significant DNA damage was obtained for the exposed group, which was associated with the pesticide exposure.
dc.description.sponsorshipCanakkale Onsekiz Mart University [2010/128]
dc.description.sponsorshipThis study was supported by Canakkale Onsekiz Mart University [Grant number: 2010/128].
dc.identifier.doi10.1080/1354750X.2019.1610498
dc.identifier.endpage599
dc.identifier.issn1354-750X
dc.identifier.issn1366-5804
dc.identifier.issue6
dc.identifier.pmid31020853
dc.identifier.scopus2-s2.0-85067875689
dc.identifier.scopusqualityQ2
dc.identifier.startpage592
dc.identifier.urihttps://doi.org/10.1080/1354750X.2019.1610498
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25073
dc.identifier.volume24
dc.identifier.wosWOS:000475147700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiomarkers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDNA damage
dc.subjectgreenhouse workers
dc.subjectpesticide
dc.subjectgenotoxicity
dc.subjectcomet assay
dc.titleComet assay for assessment of DNA damage in greenhouse workers exposed to pesticides
dc.typeArticle

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