Individual and combined inhibitory effects of methanol and toluene on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii

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dc.authoridAYDIN, GOZDE/0000-0001-5535-6053
dc.authoridInce, Orhan/0000-0001-5028-8872
dc.authoridAkyol, Cagri/0000-0002-9907-6616
dc.authoridAYMAN OZ, Nilgun/0000-0002-6309-0547
dc.authoridCetecioglu, Zeynep/0000-0002-8170-379X
dc.contributor.authorAkyol, Cagri
dc.contributor.authorInce, Orhan
dc.contributor.authorCoban, Halil
dc.contributor.authorKoksel, Gozde
dc.contributor.authorCetecioglu, Zeynep
dc.contributor.authorOz, Nilgun Ayman
dc.contributor.authorInce, Bahar
dc.date.accessioned2025-01-27T20:39:05Z
dc.date.available2025-01-27T20:39:05Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractOrganic solvents found in waste streams can be inhibitory to microbial communities in wastewater treatment plants. In this study, possible inhibitory effects of commonly used organic solvents, methanol and toluene, were investigated in batch anaerobic digestion tests. Anaerobic sludges were fed with acetate repeatedly and exposed to various concentrations of toluene, methanol and mixture of them. Expression level of the key enzyme of acetoclastic methanogenesis, acetyl-CoA synthetase 1 (Acs1), was determined by reverse transcriptase real time PCR. Additionally, active populations of Methanosaeta spp. were monitored and quantified by fluorescent in situ hybridization (FISH). Transcript abundance of Acs1 was 1.31 x 10(6) mRNAs ml(-1) in the control reactors; whereas, singular methanol exposure of 0.1 M, 0.3 M, 0.5 M and 1.0 M and methanol + toluene combination of 1.0 M + 0.5 mM and 1.0 M + 1.5 mM did not cause any significant effect on the acetyl-CoA expression level. However, singular toluene-exposed serum bottle reactors were completely inhibited after 3rd exposure at all concentrations from 0.5 mM to 4.0 mM. FISH results for singular methanol and toluene additions as well as their combination in the ranges studied showed no particular effect on the relative abundance of Methanosaeta spp. cells. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipFoundation of the Scientific and Technological Research Council of Turkey [106Y241]; Bogazici University [01M102]
dc.description.sponsorshipThis study was supported by grants from the Foundation of the Scientific and Technological Research Council of Turkey (Project No. 106Y241) and Research Fund of Bogazici University (Project No. 01M102).
dc.identifier.doi10.1016/j.ibiod.2015.09.013
dc.identifier.endpage238
dc.identifier.issn0964-8305
dc.identifier.issn1879-0208
dc.identifier.scopus2-s2.0-84942512377
dc.identifier.scopusqualityQ1
dc.identifier.startpage233
dc.identifier.urihttps://doi.org/10.1016/j.ibiod.2015.09.013
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23855
dc.identifier.volume105
dc.identifier.wosWOS:000364264300029
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofInternational Biodeterioration & Biodegradation
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAcetyl-CoA synthetase
dc.subjectFISH
dc.subjectMethanol
dc.subjectMethanosaeta concilii
dc.subjectmRNA
dc.subjectReal-time PCR
dc.subjectToluene
dc.titleIndividual and combined inhibitory effects of methanol and toluene on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii
dc.typeArticle

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