Enhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic

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dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.contributor.authorDerkus, Burak
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorEmregul, Kaan C.
dc.contributor.authorEmregul, Emel
dc.date.accessioned2025-01-27T20:41:15Z
dc.date.available2025-01-27T20:41:15Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, we describe the sonochemical isolation of nano-sized spherical hydroxyapatite (nHA) from egg shell and application towards thrombin aptasensing. In addition to the sonochemical method, two conventional methods present in literature were carried out to perform a comparative study. Various analysis methods including Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Energy Dispersive Analysis of X-Rays (EDAX), and Thermal Gravimetric Analysis (TGA) have been applied for the characterization of nHA and its nanocomposite with marine-derived collagen isolated from Rhizostoma pulmo jellyfish. TEM micrographs revealed the sonochemically synthesized nHA nanoparticles to have a unique porous spherical shape with a diameter of approximately 60-80 nm when compared to hydroxyapatite nanoparticles synthesized using the other two methods which had a typical needle shaped morphology. EDAX, XRD and FTIR results demonstrated that the obtained patterns belonged to hydroxyapatite. Electrochemical impedance spectroscopy (EIS) is the main analyzing technique of the developed thrombin aptasensor. The proposed aptasensor has a detection limit of 0.25 nM thrombin. For clinical application of the developed aptasensor, thrombin levels in blood and cerebrospinal fluid (CSF) samples obtained from patients with Multiple Sclerosis, Myastenia Gravis, Epilepsy, Parkinson, polyneuropathy and healthy donors were analyzed using both the aptasensor and commercial ELISA kit. The results showed that the proposed system is a promising candidate for clinical analysis of thrombin. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [113S255]
dc.description.sponsorshipThis work was financially supported by the Scientific and Technological Research Council of Turkey (no. 113S255), and produced from Ph.D. thesis of Burak Derkus (Ankara University Graduate School of Natural and Applied Sciences).
dc.identifier.doi10.1016/j.talanta.2016.05.045
dc.identifier.endpage109
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid27343583
dc.identifier.scopus2-s2.0-84975760697
dc.identifier.scopusqualityQ1
dc.identifier.startpage100
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2016.05.045
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24082
dc.identifier.volume158
dc.identifier.wosWOS:000379370400014
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectImpedance spectroscopy
dc.subjectSonochemistry
dc.subjectHydroxyapatite
dc.subjectEgg shell
dc.subjectThrombin
dc.subjectAptasensor
dc.subjectNeuroclinic
dc.titleEnhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic
dc.typeArticle

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