Detection of Citrus tristeza virus (CTV) from Satsuma Owari mandarins (Citris unshiu) by direct tissue blot immunoassay (DTBIA), DAS-ELISA, and biological indexing

Basit Öğe Kaydı
dc.authoridBozan, Orhan/0000-0002-3274-5459
dc.contributor.authorKorkmaz, S.
dc.contributor.authorCevik, B.
dc.contributor.authorOnder, S.
dc.contributor.authorKoc, K.
dc.contributor.authorBozan, O.
dc.date.accessioned2025-01-27T21:13:20Z
dc.date.available2025-01-27T21:13:20Z
dc.date.issued2008
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThree different citrus-producing regions of Turkey-Edremit Gulf, Coastal Aegean, and eastern Black Sea-were surveyed in 2005 and 2006. A total of 119 samples were collected from Satsuma Owari mandarin (Citrus unshiu) trees grafted on Citrus tristeza virus (CTV)-resistant Poncirus trifoliata rootstocks in commercial groves and home gardens. All samples were tested for the presence of CTV by direct tissue blot immunoassay (DTBIA) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using young shoots. The samples that tested positive were indexed for biological properties. The results obtained from DTBIA tests showed that 20 of 119 (16.8%) tested trees were infected with CTV and 99 trees were virus free. All samples that tested positive in DTBIA were also positive in DAS-ELISA. After biological indexing, none of these 20 isolates showed any symptoms on sour orange, grapefruit, and sweet orange plants, but all isolates induced vein-clearing symptoms on Mexican lime (Citrus aurantifoli) in 9 months. Some isolates also caused leaf-cupping and chlorosis in Mexican lime. However, 15 months after initial grafting, all isolates induced mild to moderate stem-pitting on Mexican lime. In addition, young tissues, petioles, and leaf samples were collected periodically at monthly intervals for I year (2006) from a 25-year-old Satsuma Owari mandarin grafted on P. trifoliata in the Edremit Gulf. CTV was readily detected in tissue blots of young shoots and petioles from CTV-infected plants during the autumn, winter, and spring seasons. Similarly, the highest ELISA values were obtained in April; the lowest values were noted in September. This study showed that DTBIA is a rapid, sensitive, and reliable procedure for detecting CTV under field conditions.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [1040129]
dc.description.sponsorshipThis work was supported by Grant no. 1040129 from the Scientific and Technological Research Council of Turkey (TUBITAK).
dc.identifier.doi10.1080/01140670809510240
dc.identifier.endpage246
dc.identifier.issn0114-0671
dc.identifier.issn1175-8783
dc.identifier.issue4
dc.identifier.scopus2-s2.0-58149154829
dc.identifier.scopusqualityQ2
dc.identifier.startpage239
dc.identifier.urihttps://doi.org/10.1080/01140670809510240
dc.identifier.urihttps://hdl.handle.net/20.500.12428/28384
dc.identifier.volume36
dc.identifier.wosWOS:000262767600003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofNew Zealand Journal of Crop and Horticultural Science
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectCitrus tristeza virus
dc.subjectbiological indexing
dc.subjectDAS-ELISA
dc.subjectDTBIA
dc.subjectSatsuma Owari mandarin
dc.titleDetection of Citrus tristeza virus (CTV) from Satsuma Owari mandarins (Citris unshiu) by direct tissue blot immunoassay (DTBIA), DAS-ELISA, and biological indexing
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu