The Importance of Cited-1 and HIF-1? Immune Activity of Granulosa Cells in IVF Treatment

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dc.contributor.authorSarı, Umut
dc.contributor.authorÖzdemir, İlhan
dc.contributor.authorBaş, Dilek Doğan
dc.contributor.authorÖztürk, Şamil
dc.date.accessioned2025-01-27T19:34:48Z
dc.date.available2025-01-27T19:34:48Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAim: The aim of this study was to investigate the Cited-1 and HIF-1? immune activity in granulosa cells in follicular development in patients who underwent IVF for infertility. Materials and Methods: This study was conducted on 40 patients who were admitted to the assisted reproductive program with the complaint of infertility at the Gazi Yaşargil Training and Research Hospital Obstetrics and Gynecology Clinic IVF center between January 2022 and November 2022 and had primary or secondary infertility while starting the Ovum-Pick-Up (OPU) procedure. The fluid containing the granulosa cells was centrifuged at 3000 rpm for 10 min. The samples were fixed and processed for routine paraffine wax tissue embedding protocol. Sections were taken from paraffin blocks and immune stained with Cited-1 and HIF-1?. The preparations were examined under the microscope. Results: HIF-1? expression was positive in membrane of granulosa cells. The nuclei were apoptotic and pyknotic. Cited1 expression was positive in membrane of granulosa cells. the cells were pyknotic. Conclusion: The high level of HIF-1? immunopositivity and negative Cited1 immunoreactivity in the immunohistochemical staining after the granulosa cells around the oocytes collected from female patients admitted to the IVF clinic and diagnosed with infertility showed that granulosa cell viability may be important on oocyte quality.
dc.identifier.doi10.36516/jocass.1491971
dc.identifier.endpage135
dc.identifier.issn2667-498X
dc.identifier.issue3
dc.identifier.startpage132
dc.identifier.trdizinid1271165
dc.identifier.urihttps://doi.org/10.36516/jocass.1491971
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1271165
dc.identifier.urihttps://hdl.handle.net/20.500.12428/16718
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofÇukurova Anestezi ve Cerrahi Bilimler Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectInfertility
dc.subjectHIF-1?
dc.subjectOocyte
dc.subjectCited-1
dc.subjectGranulosa cells
dc.titleThe Importance of Cited-1 and HIF-1? Immune Activity of Granulosa Cells in IVF Treatment
dc.typeArticle

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