Photoamperometric flow injection analysis of glucose based on dehydrogenase modified quantum dots-carbon nanotube nanocomposite electrode
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Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this work, a core-shell quantum dot (QD, ZnS-CdS) was electrodeposited onto multiwalled carbon nano tube modified glassy carbon electrode (ZnS-CdS/MWCNT/GCE) and following glucose dehydrogenase (GDH) was immobilized onto QD modified electrode. The proposed electrode (GDH/ZnS-CdS/MWCNT/ GCE) was effectively used for the photoelectrochemical biosensing of glucose in flow injection analysis (FIA) system using a home-made flow cell. Results from cyclic voltammetric and FI amperometric measurements have revealed that GDH/ZnS-CdS/MWCNT/GCE is capable of signaling photoelectrocatalytic activity toward NADH when the surface of enzyme modified electrode was irradiated with a light source (250W Halogen lamp). Thus, photoelectrochemical biosensing of glucose was monitored by recording current-time curve of enzymatically produced NADH at optimized conditions. The biosensor response was found linear over the range 0.010-2.0 mM glucose with detection limits of 6.0 and 4.0 mu M for amperometric and photoamperometric methods, respectively. The relative standard deviations (n = 5) for 0.5 mM glucose were 5.8% and 3.8% for photoamperometric and amperometric results, respectively. The photoelectrochemical biosensor was successfully applied to the real samples. The results with this biosensor showed good selectivity, repeatability and sensitivity for monitoring glucose in amperometric and photoamperometric HA studies. (C) 2016 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Glucose dehydrogenase, ZnS-CdS quantum dots, Flow injection analysis, Photoelectrochemical biosensor, NADH, Multiwalled carbon nanotube
Kaynak
Bioelectrochemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
112
