Reduction Behavior of Moxifloxacin Hydrocholaride and Its Analysis in Spiked Human Urine and Dosage Form by Voltammetry

Avoltammetric method was developed to analyze moxifloxacin hydrochloride (Mox- HCl) in pharmaceutical preparations and spiked human urine. The experimental measurements were completed using different buffer systems (pH 0.50- 12.05) on Glassy Carbon Electrode (GCE) with differential pulse voltammetry (DPV) and Cyclic Voltammetry (CV) methods. The effect of pH on the peak current and potential were determined. Britton-Robinson (BR) buffer (pH 7.00) was used for analysis. The peak was established to be diffusion-controlled based on the nature of electrode. Calibration plots were prepared for the concentration range from 3×10-4 M to 1.1×10-3 M with DPV. Limits of detection (LOD) and quantification (LOQ) were calculated as 5.1×10-5 M and 2.30×10-4 M, respectively. The validation of the applied methods was completed. Electroreduction mechanism was proposed.