Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

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dc.contributor.authorKaya, Çağlar
dc.contributor.authorSarıyer, Tolga
dc.contributor.authorŞahin, Esra
dc.date.accessioned2025-01-27T19:30:46Z
dc.date.available2025-01-27T19:30:46Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGiant Snowdrop (Galanthus elwesii Hook) is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as % (percent). For sterilization G. elwesii bulbs were sterilized at different concentrations (1, 3, 5, 7, 9, 11 and 13 %) of sodium hypochlorite (NaOCl) for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent (%) after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3% sodium hypochlorite solution however 100% contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11% sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.
dc.identifier.doi10.31015/jaefs.2023.2.12
dc.identifier.endpage348
dc.identifier.issn2602-246X
dc.identifier.issn2618-5946
dc.identifier.issue2
dc.identifier.startpage345
dc.identifier.trdizinid1183965
dc.identifier.urihttps://doi.org/10.31015/jaefs.2023.2.12
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1183965
dc.identifier.urihttps://hdl.handle.net/20.500.12428/16249
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofInternational Journal of Agriculture, Environment and Food Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectBiyoloji
dc.subjectBahçe Bitkileri
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.titleDetermination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs
dc.typeArticle

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