In vitro effect of different Na+/K+ ratios on plasma membrane H+-ATPase activity in maize and sugar beet shoot

Basit Öğe Kaydı
dc.authoridWakeel, Abdul/0000-0001-6521-6011
dc.contributor.authorWakeel, Abdul
dc.contributor.authorSuemer, Ali
dc.contributor.authorHanstein, Stefan
dc.contributor.authorYan, Feng
dc.contributor.authorSchubert, Sven
dc.date.accessioned2025-01-27T20:56:06Z
dc.date.available2025-01-27T20:56:06Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPlant growth is impaired primarily by osmotic stress in the first phase of salt stress, whereas Na+ toxicity affects the plant growth mainly in the second phase. Salinity leads to increased Na+/K+ ratio and thus displacement of K+ by Na+ in the plant cell. Relatively higher cytosolic Na+ concentrations may have an effect on the activity of plasma membrane (PM) H+-ATPase. A decreased PM-H+-ATPase activity could increase the apoplastic pH. This process could limit the cell-wall extensibility and thus reduce growth according to the acid growth theory. To compare the effect of Na+ on PM H+-ATPase activity in salt-sensitive maize (Zea mays L) and salt-resistant sugar beet (Beta vulgaris L) shoot, PM vesicles were isolated from growing shoots of both species and ATPase activity was determined by assaying the P-i released by hydrolysis of ATP. The H+ pumping activity was measured as the quenching of acridine-orange absorbance. An increased Na+/K+ ratio decreased the PM H+-ATPase activity in vesicles of maize as well as of sugar beet shoots. Nevertheless, the detrimental effect of increased Na+/K+ ratio was more severe in salt-sensitive maize compared to salt-resistant sugar beet. At 25 mM Na+ concentration, hydrolytic activity was not affected in sugar beet. However, a significant decrease in hydrolytic activity was observed in maize at pH 7. In maize and sugar beet, reduction in active H+ flux was 20% and 5% at 25 mM Na+ concentration in the assay, respectively. The active H+ flux was decreased to 80% and 60%, when 100 mM K+ were substituted by 100 mM Na+. We conclude that PM H+-ATPases of salt-resistant sugar beet and maize shoot are sensitive to higher concentration of Na+. However, sugar beet PM-H+-ATPases are relatively efficient and may have constitutive resistance against lower concentration (25 mM) of Na+ as compared to that of salt-sensitive maize. (C) 2011 Elsevier Masson SAS. All rights reserved.
dc.identifier.doi10.1016/j.plaphy.2011.01.006
dc.identifier.endpage345
dc.identifier.issn0981-9428
dc.identifier.issue3
dc.identifier.pmid21282062
dc.identifier.scopus2-s2.0-79951675826
dc.identifier.scopusqualityQ1
dc.identifier.startpage341
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2011.01.006
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26306
dc.identifier.volume49
dc.identifier.wosWOS:000288777300014
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.relation.ispartofPlant Physiology and Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBeta vulgaris
dc.subjectNa+ toxicity
dc.subjectProton pump
dc.subjectSalt stress
dc.subjectZea mays
dc.titleIn vitro effect of different Na+/K+ ratios on plasma membrane H+-ATPase activity in maize and sugar beet shoot
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu