Supercritical CO2-Mediated Decellularization of Bovine Spinal Cord Meninges: A Comparative Study for Decellularization Performance

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dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridKurt, Tugce/0000-0002-9471-9918
dc.authoridCengiz, Ugur/0000-0002-0400-3351
dc.authoridOzudogru, Eren/0000-0002-6442-7842
dc.contributor.authorOzudogru, Eren
dc.contributor.authorKurt, Tugce
dc.contributor.authorDerkus, Burak
dc.contributor.authorCengiz, Ugur
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2025-01-27T20:17:19Z
dc.date.available2025-01-27T20:17:19Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe extracellular matrix (ECM) of spinal meninge tissue closely resembles the wealthy ECM content of the brain and spinal cord. The ECM is typically acquired through the process of decellularizing tissues. Nevertheless, the decellularization process of the brain and spinal cord is challenging due to their high-fat content, in contrast to the spinal meninges. Hence, bovine spinal cord meninges offer a promising source to produce ECM-based scaffolds, thanks to their abundance, accessibility, and ease of decellularization for neural tissue engineering. However, most decellularization techniques involve disruptive chemicals and repetitive rinsing processes, which could lead to drastic modifications in the tissue ultrastructure and a loss of mechanical stability. Over the past decade, supercritical fluid technology has experienced considerable advancements in fabricating biomaterials with its applications spreading out to tissue engineering to tackle the complications mentioned above. Supercritical carbon-dioxide (scCO2)-based decellularization procedures especially offer a significant advantage over classical decellularization techniques, enabling the preservation of extracellular matrix components and structures. In this study, we decellularized the bovine spinal cord meninges by seven different methods. To identify the most effective approach, the decellularized matrices were characterized by dsDNA, collagen, and glycosaminoglycan contents and histological analyses. Moreover, the mechanical properties of the hydrogels produced from the decellularized matrices were evaluated. The novel scCO2-based treatment was completed in a shorter time than the conventional method (3 versus 7 days) while maintaining the structural and mechanical integrity of the tissue. Additionally, all hydrogels derived from scCO2-decellularized matrices demonstrated high cell viability and biocompatibility in a cell culture. The current study suggests a rapid, effective, and detergent-free scCO2-assisting decellularization protocol for clinical tissue engineering applications.
dc.description.sponsorship?anakkale Onsekiz Mart ?niversitesi [2022-B-03-24533]; Health Institutes of Turkiye [FDK-2024-4659]; Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit
dc.description.sponsorshipWe thank the staff of Canakkale Onsekiz Mart University Science and Technology Application and Research Center (COBILTUM) for their technical assistance. This work was supported by the Health Institutes of Turkiye (TUSEB) (Project ID: 2022-B-03-24533) and Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (Project ID: FDK-2024-4659). We also thank Mr. Yucel Okatali (MER-TER Medikal) for his assistance in histological studies.
dc.identifier.doi10.1021/acsomega.4c08684
dc.identifier.endpage48790
dc.identifier.issn2470-1343
dc.identifier.issue49
dc.identifier.pmid39676980
dc.identifier.scopus2-s2.0-85210298649
dc.identifier.scopusqualityQ1
dc.identifier.startpage48781
dc.identifier.urihttps://doi.org/10.1021/acsomega.4c08684
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21547
dc.identifier.volume9
dc.identifier.wosWOS:001362809500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Omega
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectCarbon-Dioxide
dc.subjectCollagen
dc.subjectMatrix
dc.subjectBiomaterials
dc.subjectProgress
dc.subjectTissues
dc.titleSupercritical CO2-Mediated Decellularization of Bovine Spinal Cord Meninges: A Comparative Study for Decellularization Performance
dc.typeArticle

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