N-glycans from human milk glycoproteins are selectively released by an infant gut symbiont in vivo

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dc.authoridKaplan, Merve/0000-0002-3322-0988
dc.authoridFrese, Steven/0000-0003-2053-5830
dc.authoridKARAV, SERCAN/0000-0003-4056-1673
dc.contributor.authorKarav, Sercan
dc.contributor.authorCasaburi, Giorgio
dc.contributor.authorArslan, Aysenur
dc.contributor.authorKaplan, Merve
dc.contributor.authorSucu, Berfin
dc.contributor.authorFrese, Steven
dc.date.accessioned2025-01-27T20:16:59Z
dc.date.available2025-01-27T20:16:59Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractComplex, indigestible free oligosaccharides as well as conjugated glycans are found in milk that shape the gut microbiome of infants. The activity of an endo-beta-N-acetylglucosaminidase from B. longum subsp. infantis (B. infantis) is known to release N-glycans from native milk glycoproteins under physiological conditions. We investigated whether this enzyme is active in vivo in breastfed infants fed B. infantis EVC001. Using mass spectrometry, we found 19 N-glycans related to human milk glycoproteins increased in abundance, similar to previous work using bovine milk glycoproteins, and these 19 N-glycans matched unique specificities of this enzyme. Twenty N-glycans were unique to infants fed B. infantis EVC001. Bifidobacteriaceae were correlated with these glycans, confirming the relationship between B. infantis and released N-glycans. This suggests that this enzyme is active in vivo and releases N-glycans from milk glycoproteins, and may play a role in B. infantis EVC001 colonization of the gut microbiome.
dc.description.sponsorshipEvolve Biosystems, Inc.
dc.description.sponsorshipSpectra were collected in the laboratory of Carlito Lebrilla, to whom the authors are extremely grateful. This work was funded by Evolve Biosystems, Inc.
dc.identifier.doi10.1016/j.jff.2019.103485
dc.identifier.issn1756-4646
dc.identifier.scopus2-s2.0-85070716976
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jff.2019.103485
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21455
dc.identifier.volume61
dc.identifier.wosWOS:000485205700036
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Functional Foods
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectProbiotic
dc.subjectMicrobiome
dc.subjectHuman milk
dc.subjectGlycoproteins
dc.subjectMilk
dc.subjectBifidobacterium infantis
dc.titleN-glycans from human milk glycoproteins are selectively released by an infant gut symbiont in vivo
dc.typeArticle

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