Photoelectrochemical glucose biosensor based on a dehydrogenase enzyme and NAD+/NADH redox couple using a quantum dot modified pencil graphite electrode

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.contributor.authorErtek, Bensu
dc.contributor.authorAkgul, Cahit
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:20:16Z
dc.date.available2025-01-27T20:20:16Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA simple, disposable and economical modified electrode was prepared by electrodeposition of hybrid quantum dots (ZnS-CdS) onto a pencil graphite electrode (PGE) surface and subsequent immobilization of glucose dehydrogenase (GDH) onto the quantum dot modified electrode (GDH/ZnS-CdS/PGE). The prepared electrode was effectively used for the photoelectrochemical determination of glucose in a flow injection analysis (FIA) system using a new home-made flow cell which was designed for PGEs for the first time. Results from the cyclic voltammetric and FI amperometric measurements revealed that the GDH/ZnS-CdS/PGE is capable of signaling photoelectrocatalytic activity involving NADH when the surface of the GDH/ZnSr-CdS/PGE is irradiated with a light source with a fiber optic cable (250 W halogen lamp). The currents of NADH produced by the enzymatic reaction in the photoamperometric FIA system under optimized conditions (carrier stream: 0.1 M phosphate buffer solution (pH 7.0) containing 1.0 M KCl and 10.0 mM NAD(+), applied potential: +0.8 V vs. Ag/AgCl/KCl(sat.); flow rate: 0.6 mL min(-1), sample loop: 100 mu L; transmission tubing length: 10 cm) were linearly correlated with the glucose concentration. Calibration curves were obtained for glucose concentrations within a range from 0.2 to 8.0 mM. The detection limits were found to be 0.09 and 0.05 mM for the amperometric and photoamperometric methods, respectively. The relative standard deviations (n = 7) for 0.5 mM glucose were 4.5% and 3.5% from the photoamperometric and amperometric results respectively. The photoelectrochemical biosensor was applied to real samples successfully. The results with this biosensor showed good selectivity, repeatability and sensitivity for monitoring glucose in amperometric and photoamperometric FIA studies.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112T375]
dc.description.sponsorshipWe thank The Scientific and Technological Research Council of Turkey (TUBITAK) for financial support (project number: 112T375).
dc.identifier.doi10.1039/c5ra25673a
dc.identifier.endpage20066
dc.identifier.issn2046-2069
dc.identifier.issue24
dc.identifier.scopus2-s2.0-84958985914
dc.identifier.scopusqualityQ1
dc.identifier.startpage20058
dc.identifier.urihttps://doi.org/10.1039/c5ra25673a
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21642
dc.identifier.volume6
dc.identifier.wosWOS:000371122900055
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofRsc Advances
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectCdse/Cds Core/Shell Nanocrystals
dc.subjectElectrocatalytic Oxidation
dc.subjectCarbon Nanotubes
dc.subjectElectrochemical Detection
dc.subjectAdenine-Dinucleotide
dc.subjectVisible Irradiation
dc.subjectBiological Samples
dc.subjectHydrogen-Peroxide
dc.subjectNadh
dc.subjectSensors
dc.titlePhotoelectrochemical glucose biosensor based on a dehydrogenase enzyme and NAD+/NADH redox couple using a quantum dot modified pencil graphite electrode
dc.typeArticle

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