A new measurement approach of ionizing radiation in irradiated trout (Oncorhynchus mykiss) by Randomly Polymorphic DNA-Polymerase Chain Reaction

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dc.contributor.authorSakalar, Ergun
dc.contributor.authorMol, Suhendan
dc.date.accessioned2025-01-27T20:54:19Z
dc.date.available2025-01-27T20:54:19Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractTrout (Oncorhynchus mykiss) were irradiated at doses of 0.250, 0.500, 1, 3, 5, 7 and 9 kGy in gamma cell. DNAs were extracted from the irradiated samples before and after storage. 1ERP primers were designed, and RAPD-PCR (Randomly Polymorphic DNA-Polymerase Chain Reaction) was applied to make randomly amplifications on the DNA of the irradiated samples. Agarose gel profiles of irradiated fish were obtained to determine change of band profiles. In addition, DNA fragmentation occurring in each dose was determined by comet assay for the verification of methodology developed in this study. The molecular methodology was developed to estimate ionizing radiation (IR) level in irradiated fish. This methodology allows the analysis of the trout irradiated up to the dose limit of around 0.5 kGy and stored for a period of three months.
dc.description.sponsorshipOffice of Scientific Research Projects at Istanbul University [18169]
dc.description.sponsorshipThe authors would like to thank the Office of Scientific Research Projects at Istanbul University for its financial support to this proejct with the Grant No. 18169, and the Biology Department of Fatih University, which provided necessary facilities.
dc.identifier.doi10.1007/s13197-015-2168-6
dc.identifier.endpage2496
dc.identifier.issn0022-1155
dc.identifier.issn0975-8402
dc.identifier.issue5
dc.identifier.pmid27407216
dc.identifier.scopus2-s2.0-84964328665
dc.identifier.scopusqualityQ1
dc.identifier.startpage2492
dc.identifier.urihttps://doi.org/10.1007/s13197-015-2168-6
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26044
dc.identifier.volume53
dc.identifier.wosWOS:000380102100036
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer India
dc.relation.ispartofJournal of Food Science and Technology-Mysore
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectOncorhynchus mykiss
dc.subjectIrradiation dose
dc.subjectPCR
dc.subjectComet assay
dc.subjectDNA damage
dc.titleA new measurement approach of ionizing radiation in irradiated trout (Oncorhynchus mykiss) by Randomly Polymorphic DNA-Polymerase Chain Reaction
dc.typeArticle

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