Inhibition effect of isopropanol on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii

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dc.authoridAYMAN OZ, Nilgun/0000-0002-6309-0547
dc.authoridCetecioglu, Zeynep/0000-0002-8170-379X
dc.authoridAYDIN, GOZDE/0000-0001-5535-6053
dc.authoridInce, Orhan/0000-0001-5028-8872
dc.contributor.authorInce, Bahar
dc.contributor.authorKoksel, Gozde
dc.contributor.authorCetecioglu, Zeynep
dc.contributor.authorOz, Nilgun Ayman
dc.contributor.authorCoban, Halil
dc.contributor.authorInce, Orhan
dc.date.accessioned2025-01-27T21:01:39Z
dc.date.available2025-01-27T21:01:39Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIsopropanol is a widely found solvent in industrial wastewaters, which have commonly been treated using anaerobic systems. In this study, inhibitory effect of isopropanol on the key microbial group in anaerobic bioreactors, acetoclastic methanogens, was investigated. Anaerobic sludges in serum bottles were repeatedly fed with acetate and isopropanol; and quantitative real-time PCR was used for determining effect of isopropanol on the expression level of a key enzyme in acetoclastic methane production, acetyl-CoA synthetase of Methanosaeta concilii. Active Methanosaeta spp. cells were also quantified using Fluorescent in situ hybridization (FISH). Transcript abundance of acetyl-CoA synthetase was 1.23 +/- 0.62 x 10(6) mRNAs/mL in the uninhibited reactors with 222 mL cumulative methane production. First exposure to isopropanol resulted in 71.2%, 84.7%, 89.2% and 94.6% decrease in mRNA level and 35.0%, 65.0%, 91.5% and 100.0% reduction in methane production for isopropanol concentrations of 0.1 M, 0.5 M, 1.0 M and 2.0 M, respectively. Repeated exposures resulted in higher inhibitions; and at the end of test, fluorescent intensities of active Methanosaeta cells were significantly decreased due to isopropanol. The overall results indicated that isopropanol has an inhibitory effect on acetoclastic methanogenesis; and the inhibition can be detected by monitoring level of acetyl-CoA transcripts and rRNA level. (C) 2011 Elsevier B. V. All rights reserved.
dc.description.sponsorshipFoundation of the Scientific and Technological Research Council of Turkey [106Y241]; Bogazici University [01M102]
dc.description.sponsorshipThis work was supported by grants from the Foundation of the Scientific and Technological Research Council of Turkey (Project No. 106Y241), and Research Fund of Bogazici University (Project No. 01M102).
dc.identifier.doi10.1016/j.jbiotec.2011.08.021
dc.identifier.endpage99
dc.identifier.issn0168-1656
dc.identifier.issue2
dc.identifier.pmid21884734
dc.identifier.scopus2-s2.0-80054014593
dc.identifier.scopusqualityQ1
dc.identifier.startpage95
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2011.08.021
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27140
dc.identifier.volume156
dc.identifier.wosWOS:000296528600002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal of Biotechnology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectIsopropanol
dc.subjectAcetyl-CoA synthetase
dc.subjectmRNA
dc.subjectReal-time PCR
dc.subjectFISH
dc.subjectMethanosaeta concilii
dc.titleInhibition effect of isopropanol on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii
dc.typeArticle

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