Humic Acid Has Protective Effect on Gastric Ulcer by Alleviating Inflammation in Rats

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dc.authoridOztopuz, Ozlem/0000-0002-1373-6311
dc.authoriduzun, metehan/0000-0003-1406-5473
dc.contributor.authorSehitoglu, Muserref Hilal
dc.contributor.authorOztopuz, Ozlem
dc.contributor.authorKaraboga, Ihsan
dc.contributor.authorOvali, Mehmet Akif
dc.contributor.authorUzun, Metehan
dc.date.accessioned2025-01-27T20:49:42Z
dc.date.available2025-01-27T20:49:42Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe new agents are needed in treatment of gastric ulcer that have less side effects, adequate efficacy, and no drug interactions. In this study, we aimed to investigate the potential protective effects of humic acid on experimental gastric ulcer. Wistar Albino male rats (n = 48) were randomly divided into 8 groups as follow; Control (without any applications), Humic acid (50 mg/kg), ethanol group (1 mL/rat), and indomethacin group (25 mg/kg). In the treatment groups, both gastric ulcer model and humic acid 50 mg/kg were applied. In addition, famotidine the antiulcer drug was used as positive control. All medications were administered by oral gavage. Levels of ADAM10 and ADAMTS12 in gastric mucosa were determined by ELISA method. Hematoxylin-Eosin (H&E) staining, iNOS, and PCNA immunohistochemical staining were performed for histopathological investigations. Apoptosis was demonstrated by using the TUNEL method. In addition, the levels of inflammatory cytokines (TNF-alpha, IL-6, IL-10) and caspase-3 gene were determined by qRT-PCR. ADAM10 and ADAMTS12 levels significantly increased in the treatment groups compared to the ulcer groups (p < 0.05). The experimental groups showed mucosal erosion, bleeding, leukocyte infiltration and edema. Treatment with humic acid and famotidine was found to suppress iNOS activity, thereby decreasing proinflammatory activity and preventing damage to the gastric mucosa, while reducing the number of apoptotic cells. IL-6, IL-10, TNF-alpha and caspase-3 levels were significantly decreased in the treatment groups compared to damaged gastric mucosa. As a result, humic acid may be defined as a potential protective agent with its anti-inflammatory effect in gastric ulcer.
dc.description.sponsorshipScientific Research Commission of Canakkale Onsekiz Mart University with the project named Investigation of the Effects of Humic Acid in Different Acute Gastric Ulcer Models in Rats [TSA-2018-2592]
dc.description.sponsorshipThis study was supported by the Scientific Research Commission of Canakkale Onsekiz Mart University with the project named Investigation of the Effects of Humic Acid in Different Acute Gastric Ulcer Models in Rats coded TSA-2018-2592.
dc.identifier.doi10.3103/S0095452722010091
dc.identifier.endpage97
dc.identifier.issn0095-4527
dc.identifier.issn1934-9440
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85124947531
dc.identifier.scopusqualityQ3
dc.identifier.startpage84
dc.identifier.urihttps://doi.org/10.3103/S0095452722010091
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25285
dc.identifier.volume56
dc.identifier.wosWOS:000754205100010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPleiades Publishing Inc
dc.relation.ispartofCytology and Genetics
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectKeywords
dc.subjectgastric mucosa injury
dc.subjecthumic acid
dc.subjectADAM10
dc.subjectADAMTS12
dc.subjectinflammation
dc.subjectfamotidine
dc.titleHumic Acid Has Protective Effect on Gastric Ulcer by Alleviating Inflammation in Rats
dc.typeArticle

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