Highly sensitive electrochemical immunosensor based on polythiophene polymer with densely populated carboxyl groups as immobilization matrix for detection of interleukin 1? in human serum and saliva

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:02:05Z
dc.date.available2025-01-27T21:02:05Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA new impedimetric immunosensor was fabricated for detection of Interleukin 1 beta (IL-1 beta) by using semi-conductive poly(2-thiophen-3-yl-malonic acid) (P3-TMA) as an immobilization matrix material and anti-IL-1 beta antibody as a biorecognition element for the first time. The polymer P3-TMA bound onto hydroxylated ITO surface via ester bond to form a polymer interface including active carboxyl groups. These reactive carboxyl groups bound to anti-IL-1 beta antibodies via amide bond under coupling reagent of N-beta-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide. The detection of IL-1 beta antigen was monitored with electrochemical impedance spectroscopy technique (EIS). Apart from EIS technique, cyclic voltammetry (CV), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy dispersive X-ray (EDX) mapping, atomic force microscopy (AFM) were used for characterization of immunosensor. The effect of antibody and antigen incubation durations, and utilized biorecognition element concentrations were investigated to determine the optimum analytical performance of the immunosensor. Under optimized conditions, the relative change in impedance was proportional to the IL-1 beta concentration in the range of 0.01-3 pg/mL with the detection limit 3 fg/mL. The proposed immunosensor had good specificity, reproducibility and stability. The immunosensor was applicable for detection of IL-1 beta in human serum and saliva samples with satisfied recoveries (97.4-104.5%). (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.snb.2018.05.014
dc.identifier.endpage27
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85046625626
dc.identifier.scopusqualityQ1
dc.identifier.startpage18
dc.identifier.urihttps://doi.org/10.1016/j.snb.2018.05.014
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27275
dc.identifier.volume270
dc.identifier.wosWOS:000434011500003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectInterleukin 1 beta
dc.subjectDisposable electrode
dc.subjectIndium tin oxide
dc.subjectSaliva biomarker
dc.subjectPolythiophene
dc.titleHighly sensitive electrochemical immunosensor based on polythiophene polymer with densely populated carboxyl groups as immobilization matrix for detection of interleukin 1? in human serum and saliva
dc.typeArticle

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