Ultrasensitive and Cost-Effective Detection of Neuropeptide-Y by a Disposable Immunosensor: A New Functionalization Route for Indium-Tin Oxide Surface

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dc.authoridUludag, Inci/0000-0003-4296-2657
dc.contributor.authorUludag, Inci
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:27:23Z
dc.date.available2025-01-27T20:27:23Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractNeuropeptide Y (NPY) is one of the most abundant neuropeptides in the human brain, and its levels in the blood change in neurodegenerative and neuroimmune disorders. This indicates that NPY may serve as a diagnostic and monitoring marker for associated disorders. In this paper, an electrochemical immunosensor was created to detect NPY biomarkers using a novel immobilization technique. The proposed biosensor system enables accurate, specific, cost-effective, and practical biomarker analysis. Indium tin oxide-coated polyethylene terephthalate (ITO-PET) sheets were treated with hexamethylene diisocyanate (HMDC) to covalently immobilize antibodies. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were used to analyze each step of the biosensors. The proposed NPY biosensor has a broad linear detection range (0.01-100 pg mL(-1)), a low limit of detection (LOD) (0.02968 pg mL(-1)), and a low limit of quantification (LOQ) (0.0989 pg mL(-1)). Atomic force microscopy (AFM) was used to support in the optimization process, study the surface morphology, and visualize it. Studies of repeatability, reproducibility, storage, and Kramers-Kronig transformation were conducted during electrochemical characterization. After analytical experiments, the biosensor's responses to human serum samples were evaluated. According to the obtained data, the error margin is small, and the created biosensor offers a great deal of promise for the clinical measurement of NPY.
dc.description.sponsorshipCanakkale Onsekiz Mart University Council of Scientific Research Project [FYL-2019-3102]
dc.description.sponsorshipThis work was funded by the Canakkale Onsekiz Mart University Council of Scientific Research Project [Project number: FYL-2019-3102].
dc.identifier.doi10.3390/bios12110925
dc.identifier.issn2079-6374
dc.identifier.issue11
dc.identifier.pmid36354435
dc.identifier.scopus2-s2.0-85141767480
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/bios12110925
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22676
dc.identifier.volume12
dc.identifier.wosWOS:000880914300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofBiosensors-Basel
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectNeuropeptide Y
dc.subjectindium tin oxide-coated polyethylene terephthalate
dc.subjecthexamethylene diisocyanate
dc.subjectelectrochemical impedance spectroscopy
dc.subjectneurodegenerative disease
dc.subjectneuroimmune disorders
dc.titleUltrasensitive and Cost-Effective Detection of Neuropeptide-Y by a Disposable Immunosensor: A New Functionalization Route for Indium-Tin Oxide Surface
dc.typeArticle

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