Chitosan-Based Cryogels for Esterase Immobilization

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dc.authorid0000-0002-7712-754X
dc.contributor.authorEkici, Sema
dc.contributor.authorBaybas, Demet
dc.contributor.authorAyan, Beyza
dc.date.accessioned2026-02-03T12:03:10Z
dc.date.available2026-02-03T12:03:10Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPreparing chitosan (Ch) cryogels in the form of full-interpenetrating polymer networks (IPNs) is an advantageous approach since the IPN cryogels have a more regular and tight pore distribution and durable spongy structure, including enhanced mechanical properties and more functional groups. In the present research, Ch-Ch ([Ch](cry)) and Ch-pAAm (polyacrylamide) full-IPN cryogels ([Ch-pAAm](cry)) were synthesized, characterized, and then used for the immobilization of enzyme (porcine/pig liver esterase (PLE)) using the adsorption method. The results of mechanical analysis have shown that the compression elasticity modulus (E) value of [Ch-pAAm](cry) full-IPN cryogel is 47 times that of [Ch](cry). The introduction of pAAm within full-IPN cryogels increases the compression strength; however, it decreases their ability for esterase immobilization and swelling degrees (S-e). The esterase adsorption capacity (X-L) values are 90.7 and 13.4 mg g(-1) for [Ch](cry) and [Ch-pAAm](cry), respectively. Adsorption equilibrium time of [Ch](cry) is shorter (360 min) than [Ch-pAAm](cry) (600 min). According to K-m values, the order of affinity for the 4-nitrophenyl acetate (pNPA) substrate is [Ch-pAAm](cry) (6.9 mM) > [Ch](cry) (22.1 mM) > free enzyme (60.4 mM). The porosity parameter, V-p (cm(3) pore (g dry cryogel)(-1), was calculated to be 36.18 +/- 4.77 for [Ch](cry) and 5.00 +/- 0.76 for [Ch-pAAm](cry), indicating that [Ch](cry) had larger pores than [Ch-pAAm](cry).
dc.description.sponsorshipSivas Cumhuriyet niversitesi [F-633]
dc.description.sponsorshipThe esterase used in the study was purchased within the scope of project number F-633 of the Sivas Cumhuriyet University Scientific Research Projects Commission.
dc.identifier.doi10.1002/slct.202503526
dc.identifier.issn2365-6549
dc.identifier.issue42
dc.identifier.scopus2-s2.0-105021262898
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/slct.202503526
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34993
dc.identifier.volume10
dc.identifier.wosWOS:001610920500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistryselect
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260130
dc.subjectChitosan
dc.subjectEsterase
dc.subjectFull-IPN cryogel
dc.subjectPolyacrylamide
dc.subjectPorosity
dc.titleChitosan-Based Cryogels for Esterase Immobilization
dc.typeArticle

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