An impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth

Basit Öğe Kaydı
dc.authoridUygun, Zihni Onur/0000-0001-9045-7271
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.contributor.authorUygun, Zihni Onur
dc.date.accessioned2025-01-27T20:17:09Z
dc.date.available2025-01-27T20:17:09Z
dc.date.issued2012
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present work, we have developed a new biosensor based on fourth-generation (G4) PAMAM dendrimers for the analysis of vascular endothelial growth factor (VEGF). First, the PAMAM dendrimers were covalently attached to a cysteamine-modified Au electrode by glutaraldehyde. With the help of the amino groups located on its surface, vascular endothelial growth factor receptor-1 (VEGF-R1) was immobilized via glutaraldehyde cross-linking. VEGF-R1 loading was investigated to identify the optimal VEGF-R1 immobilization conditions for the best sensitivity of the new biosensor. In addition, Kramers-Kronig transforms were also analyzed for immobilization and measurement processes. The biosensor had a linear range of 5 to 125 pg/mL VEGF. The fabricated biosensor had good repeatability and reproducibility. Finally, the results for artificial serum samples measured by the present biosensor showed a good recovery for VEGF detection. (C) 2012 Elsevier Inc. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [109 T 172]
dc.description.sponsorshipThe authors gratefully acknowledge the support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109 T 172).
dc.identifier.doi10.1016/j.ab.2011.12.049
dc.identifier.endpage285
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.issue2
dc.identifier.pmid22266295
dc.identifier.scopus2-s2.0-84859009441
dc.identifier.scopusqualityQ2
dc.identifier.startpage277
dc.identifier.urihttps://doi.org/10.1016/j.ab.2011.12.049
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21504
dc.identifier.volume423
dc.identifier.wosWOS:000302336100014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectVEGF
dc.subjectTumor growth
dc.subjectVEGF-R1
dc.subjectPAMAM
dc.subjectElectrochemical impedance spectroscopy
dc.subjectBiosensor
dc.titleAn impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu