Detection of Kallikrein-Related Peptidase 4 with a Label-free Electrochemical Impedance Biosensor Based on a Zinc(II) Phthalocyanine Tetracarboxylic Acid-Functionalized Disposable Indium Tin Oxide Electrode
| dc.authorid | 0000-0003-3042-1087 | en_US |
| dc.authorscopusid | 35113891600 | en_US |
| dc.authorwosid | AAY-1439-2021 | en_US |
| dc.contributor.author | Aydın, Elif Burcu | |
| dc.contributor.author | Aydın, Muhammet | |
| dc.contributor.author | Yüzer, Abdülcelil | |
| dc.contributor.author | İnce, Mine | |
| dc.contributor.author | Ocakoğlu, Kasım | |
| dc.contributor.author | Sezgintürk, Mustafa Kemal | |
| dc.date.accessioned | 2023-05-10T07:32:23Z | |
| dc.date.available | 2023-05-10T07:32:23Z | |
| dc.date.issued | 2021 | en_US |
| dc.department | Fakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü | |
| dc.description.abstract | A new impedimetric biosensing system based on kallikrein-related peptidase 4 (KLK 4) antigen-specific antibodies and a zinc(II) phthalocyanine tetracarboxylic acid (Zn-PcTCa) matrix material was developed for the first time in this study. First, a Zn-PcTCa-coated indium tin oxide surface was used as an interface matrix material for the immobilization of anti-KLK 4 antibodies, and they bound to the platform via amide bonds. In the presence of KLK 4 antigens, the anti-KLK 4 antibodies specifically captured these antigens and caused changes in the electrochemical properties of the system. Randles equivalent circuit was utilized to evaluate the impedimetric signal, which was measured with the help of an electrochemical impedance spectroscopy method. After the specific interaction, the electron transfer resistance (Rct) was remarkably increased and displayed a linear relationship with the level of the KLK 4 antigen in the range of 0.02-15 pg/mL, with a a detection limit of 6.8 fg/mL. The designed biosensor was able to detect a KLK 4 antigen with good sensitivity, excellent specificity, and high stability. In addition, because of having a low-cost and robust procedure for fabrication, it could be repeatedly used in several areas including clinical diagnosis. © | en_US |
| dc.identifier.citation | Aydln, E. B., Aydln, M., Yuzer, A., Ince, M., OcakogÌlu, K., & SezgintuÌrk, M. K. (2021). Detection of kallikrein-related peptidase 4 with a label-free electrochemical impedance biosensor based on a zinc(II) phthalocyanine tetracarboxylic acid-functionalized disposable indium tin oxide electrode. ACS Biomaterials Science and Engineering, 7(3), 1192-1201. doi:10.1021/acsbiomaterials.0c01602 | en_US |
| dc.identifier.doi | 10.1021/acsbiomaterials.0c01602 | |
| dc.identifier.endpage | 1201 | en_US |
| dc.identifier.issn | 2373-9878 | |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.pmid | 33583176 | |
| dc.identifier.startpage | 1192 | en_US |
| dc.identifier.uri | https://doi.org/10.1021/acsbiomaterials.0c01602 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12428/4092 | |
| dc.identifier.volume | 7 | en_US |
| dc.identifier.wos | WOS:000627588400032 | |
| dc.identifier.wosquality | Q2 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | PubMed | |
| dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
| dc.language.iso | en | |
| dc.publisher | Amer Chemical Soc | en_US |
| dc.relation.ispartof | ACS Biomaterials Science and Engineering | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | biosensor | en_US |
| dc.subject | electrochemical impedance spectroscopy | en_US |
| dc.subject | kallikrein-related peptidase 4 | en_US |
| dc.subject | metallophthalocyanines | en_US |
| dc.subject | tetracarboxylic acid | en_US |
| dc.title | Detection of Kallikrein-Related Peptidase 4 with a Label-free Electrochemical Impedance Biosensor Based on a Zinc(II) Phthalocyanine Tetracarboxylic Acid-Functionalized Disposable Indium Tin Oxide Electrode | |
| dc.type | Article |
