Validated micellar electrokinetic capillary chromatography (MECC) method for determination of 5-hydroxymethylfurfural in honey and comparison with HPLC

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dc.authoridYILMAZ TUNCEL, NESE/0000-0003-2700-5840
dc.authoridGuray, Tufan/0000-0002-7509-7260
dc.authoridUYSAL, ULKU DILEK/0000-0003-1572-9753
dc.contributor.authorGuray, Tufan
dc.contributor.authorTuncel, Nese Yilmaz
dc.contributor.authorTuncel, Muzaffer
dc.contributor.authorUysal, Ulku Dilek
dc.date.accessioned2025-01-27T20:50:09Z
dc.date.available2025-01-27T20:50:09Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA simple and validated micellar electrokinetic chromatographic method is described for the determination of 5-hydroxymethylfurfural (HMF). The method was applied to fresh and processed (heated at 65 degrees C, 85 degrees C and 105 degrees C for 5h and 20h) multifloral and honeydew honeys. Optimum conditions were: 10mmolL(-1) Tris buffer consisting 110mmolL(-1) SDS and 10% (v/v) MeOH at pH 9.10, 20kV of applied potential, 3s of injection time at 5x10(3)Nm(-2), 280nm of wavelength and 25 degrees C of fixed temperature. Mean electrophoretic mobility (m(2)s(-1)V(-1)) of HMF and methyl paraben (IS) were -5.28x10(-5) (RSD of 0.745%) and -2.52x10(-4) (RSD of 0.944%), respectively. The calibration curve was linear in the range of 0.68 mu gmL(-1) (5.40x10(-6)molL(-1)) and 4.05 mu gmL(-1) (3.21x10(-5)molL(-1)) with R=0.9990 for inter-day precision. LOD and LOQ values were 0.007 mu gmL(-1) (5.48x10(-8)molL(-1)) and 0.023 mu gmL(-1) (1.83x10(-7)molL(-1)), respectively, as inter-day precision. The accuracy was very high with recovery values between 94.07 and 97.16%. The developed method was fully validated according to ICH guidelines. The results were compared with those of HPLC method adapted from AOAC method no: 980.23.
dc.identifier.doi10.1007/s11696-019-00770-5
dc.identifier.endpage2220
dc.identifier.issn0366-6352
dc.identifier.issn2585-7290
dc.identifier.issue9
dc.identifier.scopus2-s2.0-85067360100
dc.identifier.scopusqualityQ2
dc.identifier.startpage2209
dc.identifier.urihttps://doi.org/10.1007/s11696-019-00770-5
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25425
dc.identifier.volume73
dc.identifier.wosWOS:000471729100010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subject5-Hydroxymethylfurfural
dc.subjectHoney
dc.subjectHMF
dc.subjectCapillary electrophoresis
dc.subjectHPLC
dc.titleValidated micellar electrokinetic capillary chromatography (MECC) method for determination of 5-hydroxymethylfurfural in honey and comparison with HPLC
dc.typeArticle

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