A highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNF? in human saliva and serum samples

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:03:47Z
dc.date.available2025-01-27T21:03:47Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA novel, ultrasensitive impedimetric immunosensor was constructed for the detection of tumor necrosis factor alpha (TNF alpha) by using Poly(3-thiophene acetic acid) (P3), a conjugated polymer as an immobilization matrix. The polymer P3 contains a lot of carboxylic acid groups on its surface that provide a larger biorecognition surface. This developed immunosensor was prepared on hydroxy-bearing ITO surface via an ester bond linkage of polymer P3 to immobilize anti-TNF alpha antibodies. The ITO electrode modification steps and interaction between anti-TNF alpha antibody and TNF alpha antigen were monitored by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS) method. After the analytical parameters optimization, a linear detection response from 0.01 pg/mL to 2 pg/mL, a limit of detection LOD of 3.7 fg/mL and a limit of quantification (LOQ) of 12.4 fg/mL were achieved, which provided accurate results (relative standard deviation; 4.03%). The characterization of this developed immunosensor was performed by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy dispersive X-ray (EDX) mapping and atomic force microscopy (AFM). The immunosensor allowed a simple and fast detection of TNF alpha antigen in human serum and satisfied recoveries (98.69-105.20%) were obtained by using standard addition method.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.bios.2017.05.056
dc.identifier.endpage176
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid28599176
dc.identifier.scopus2-s2.0-85020253100
dc.identifier.scopusqualityQ1
dc.identifier.startpage169
dc.identifier.urihttps://doi.org/10.1016/j.bios.2017.05.056
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27446
dc.identifier.volume97
dc.identifier.wosWOS:000405153000025
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTumor necrosis factor alpha
dc.subjectLabel free biosensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSaliva biomarker
dc.titleA highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNF? in human saliva and serum samples
dc.typeArticle

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