In vitro activity of methylene blue and eosin methylene blue agar on colistin-resistant A. baumannii: an experimental study

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dc.authoridGazel, Deniz/0000-0003-2764-3113
dc.authoridAlper Akçalı/0000-0003-0325-886X
dc.contributor.authorGazel, Deniz
dc.contributor.authorOtkun, Muserref Tatman
dc.contributor.authorAlper Akçalı
dc.date.accessioned2025-01-27T20:11:48Z
dc.date.available2025-01-27T20:11:48Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIntroduction. Colistin is a last-resort antibiotic used against carbapenem-resistant Acinetobacter baumannii (AB); however, colistin resistance has been reported recently. Methylene blue (MB) is used in microbiology for staining, and in medicine as an antidote drug. Aim. We aimed to investigate the antimicrobial effects of MB and eosin methylene blue (EMB) agar against colistin-resistant AB strains. Methodology. Firstly, a standard strain and AB clinical isolate were included in the study. After determining MICs, two strains were transformed into colistin-resistant forms, using Li's method. At each step, new MICs were determined and subcultures were inoculated onto EMB and sheep blood agar (SBA). Colistin MICs of the subcultures were also determined using Mueller-Hinton agar (MHA) containing MB. Secondly, colistin-resistant clones from 31 multidrug-resistant AB clinical isolates were screened to investigate their susceptibilities to EMB agar. Results. In the first round, MICs of both strains had risen to 64 mu g ml(-1). Subpopulations with high colistin resistance were inhibited by MB and EMB agar, but could grow well on SBA. In MHA plates containing MB, the MICs decreased to 0.5 mu g ml(-1) for colistin-susceptible or moderately resistant clones. Additionally, clones with high colistin resistance showed atypical colony morphology on SBA. In the second round, 35% of the clinical isolates, which had gained resistance to colistin, were inhibited by EMB agar. Conclusion. MB may have inhibitory effects against colistin-resistant AB. Secondly, using only EMB agar for subculturing may cause missing of colistin-resistant strains.
dc.identifier.doi10.1099/jmm.0.001078
dc.identifier.endpage1613
dc.identifier.issn0022-2615
dc.identifier.issn1473-5644
dc.identifier.issue11
dc.identifier.pmid31535963
dc.identifier.scopus2-s2.0-85074552360
dc.identifier.scopusqualityQ2
dc.identifier.startpage1607
dc.identifier.urihttps://doi.org/10.1099/jmm.0.001078
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20735
dc.identifier.volume68
dc.identifier.wosWOS:000494937800006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMicrobiology Soc
dc.relation.ispartofJournal of Medical Microbiology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAcinetobacter baumannii
dc.subjectcolistin
dc.subjectresistant
dc.subjectmethylene blue
dc.subjectEMB
dc.titleIn vitro activity of methylene blue and eosin methylene blue agar on colistin-resistant A. baumannii: an experimental study
dc.typeArticle

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