Precision, repeatability and representative ability of faecal egg counts in Heterakis gallinarum infected chickens

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dc.authoridSavaş, Türker/0000-0002-3558-2296
dc.authoridDas, Gurbuz/0000-0001-6690-0050
dc.authoridGauly, Matthias/0000-0003-4212-5437
dc.contributor.authorDas, Guerbuez
dc.contributor.authorSavaş, Türker
dc.contributor.authorKaufmann, Falko
dc.contributor.authorIdris, Ahmad
dc.contributor.authorAbel, Hansjörg
dc.contributor.authorGauly, Matthias
dc.date.accessioned2025-01-27T20:58:08Z
dc.date.available2025-01-27T20:58:08Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study investigated whether a precise and repeatable quantification of Heterakis gallinarum egg excretion, which considerably reflects the actual worm burdens, can be achieved based on collection of the daily total amount of faeces from chickens. Three-week-old birds (N = 64) were infected with 200 embryonated eggs of H. gallinarum, and placed into individual cages 3 wk after infection for 5 wk to collect daily faeces (N = 2240). The total daily faeces was mixed and a randomly taken sample per bird was analyzed to estimate the numbers of eggs per gram of faeces (EPG) and total number of eggs excreted within 24h (EPD). A total of 235 daily faecal collections were randomly selected and further examined to determine between and within sample variations of EPG counts as a measure of precision. For this, two random faecal samples were taken from the daily produced faeces by a bird, and the EPG was determined for each of the samples (EPG1 and EPG2). The second faecal sample was analyzed once more to determine a parallel EPG2 count (EPG2a) of the suspended sample. Precision of an EPG count was defined as its relative closeness to the average of two EPG counts using a relative asymmetry index (Index(EPG)). At an age of 11 wk, i.e. 8 wk p.i. the birds were slaughtered and their worm burdens were determined. There were no significant differences between EPG1 and EPG2 (P = 0.764) nor between EPG2 and EPG2a (P = 0.700), suggesting that the differences between or within the samples were not different from zero. Correlations between EPG counts, as between and within sample coherences, were r = 0.85 and r = 0.86, respectively. Precision of EPG counts, as measured by Index(EPG), was not influenced by consistency (P = 0.870) and total amount of faeces (P = 0.088). However, concentration of eggs in faeces (mean EPG) had a significant effect on the precision of the EPG counts (P < 0.001). Similar results were also observed for the within sample precision (Index(EPG2)). A segmented regression analysis indicated an abrupt change in the precision of EPG counts as the response to changing egg concentration in the examined faecal samples. The precision of analyses remarkably heightened up to a breakpoint with an EPG count of <= 617. A similar breakpoint was also determined for within sample precision (EPG2 <= 621). Moderate repeatabilities (R = 0.49) for EPG and EPD were estimated in the first week of egg excretion, whereas the estimates were higher (R = 0.67-0.84) in the following weeks. Correlations between number of female worms with daily measured EPG and EPD increased to an almost constant level (r >= 0.70; P < 0.05) in a few days after the nematode excreted eggs and predominantly remained so for the rest of the sampling period. It is concluded that mixing daily total faeces provides samples with random homogenous distribution of H. gallinarum eggs. Precision of the EPG counts increases as the egg concentration in faecal sample increases. Egg excretion of H. gallinarum, quantified either as EPG or EPD, is highly repeatable and closely correlated with the actual worm burden of birds starting as early as in 5th wk of infection. (C) 2011 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.vetpar.2011.07.005
dc.identifier.endpage94
dc.identifier.issn0304-4017
dc.identifier.issue1-2
dc.identifier.pmid21802853
dc.identifier.scopus2-s2.0-82755182860
dc.identifier.scopusqualityQ1
dc.identifier.startpage87
dc.identifier.urihttps://doi.org/10.1016/j.vetpar.2011.07.005
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26618
dc.identifier.volume183
dc.identifier.wosWOS:000298450400014
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofVeterinary Parasitology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHeterakis gallinarum
dc.subjectPrecision
dc.subjectRepeatability
dc.subjectNematode egg shedding
dc.subjectFaecal egg counts
dc.subjectFaeces
dc.titlePrecision, repeatability and representative ability of faecal egg counts in Heterakis gallinarum infected chickens
dc.typeArticle

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