Antimicrobial activity and biocompatibility of slow-release hyaluronic acid-antibiotic conjugated particles

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dc.authoridSağbaş Suner, Selin / 0000-0002-3524-0675
dc.authoridŞahiner, Nurettin / 0000-0003-0120-530X
dc.contributor.authorZhang, Ze
dc.contributor.authorSağbaş Suner, Selin
dc.contributor.authorBlake, Diane A.
dc.contributor.authorAyyala, Ramesh S.
dc.contributor.authorŞahiner, Nurettin
dc.date.accessioned2025-01-27T20:52:11Z
dc.date.available2025-01-27T20:52:11Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHere, the aim was to design and use a long-lasting antibiotic release system for prevention of postoperative infections in ophthalmic surgery. Ciprofloxacin and vancomycin-conjugated hyaluronic acid (HA) particles were prepared as drug carriers for sustained release of antibiotics. The antimicrobial effects of the released drugs were determined by disc-diffusion and macro-dilution tests at different times up to 2 weeks. Slow degradable HA particles were obtained with 35.2 wt% degradation within 21 days. The drug loading amount was increased by employing two sequential chemical linking (conjugation, 2C) and one physical absorption loading (A) procedures (2C + A processes) from 148 +/- 8 to 355 +/- 11 mg/g HA particles for vancomycin. The amounts of vancomycin and ciprofloxacin that were released linearly was estimated as 64.35 +/- 7.35 and 25.00 +/- 0.68 mg/g, respectively, from drug-conjugated HA particles in 100 h. Antimicrobial studies revealed that antibiotic-conjugated HA particles could inhibit the growth of microorganisms from 1 h to 1 week. The MBC values were measured as 0.25, 4.0, and 0.25 mg/mL against Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, respectively, after 72 h incubation time. Cytotoxicity studies showed no difference between fibroblast growth or corneal thickness after 5 days with or without HA-antibiotic particles. The drug release studies and antimicrobial activity of antibiotic-loaded HA particles with time against various bacteria further revealed that HA particles are very effective in preventing bacterial infections. Likewise, cytotoxicity studies suggest that these particles pose no toxicity to eukaryotic cells, including corneal endothelium.
dc.identifier.doi10.1016/j.ijpharm.2020.119024
dc.identifier.issn0378-5173
dc.identifier.issn1873-3476
dc.identifier.pmid31926974
dc.identifier.scopus2-s2.0-85078146701
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijpharm.2020.119024
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25689
dc.identifier.volume576
dc.identifier.wosWOS:000512972500038
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Pharmaceutics
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHyaluronic acid microgel/nanogel
dc.subjectOphthalmic drug delivery
dc.subjectAntibiotic release system
dc.titleAntimicrobial activity and biocompatibility of slow-release hyaluronic acid-antibiotic conjugated particles
dc.typeArticle

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