Isolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soil

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dc.authoridKantar, Cetin/0000-0001-9747-9115
dc.authoridkuce cevik, pinar/0000-0001-6722-6096
dc.authoridCoral, Gokhan/0000-0002-3533-118X
dc.contributor.authorCevik, P. K.
dc.contributor.authorEroglu, A. B.
dc.contributor.authorYildizli, G.
dc.contributor.authorCosan, D.
dc.contributor.authorKantar, C.
dc.contributor.authorCoral, G.
dc.date.accessioned2025-01-27T20:16:35Z
dc.date.available2025-01-27T20:16:35Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAim : The aim of this study was to isolate, identify and characterize diethyl phthalate degrading bacterial strains isolated from a crude oil contaminated soil from a landfill dump site of a petroleum refinery in Mersin, Turkey. Methodology : The bacteria was isolated from a crude oil contaminated soil and characterized by 16S rRNA analysis. Bacterial genomic DNA was identified by 16S rRNA analyses. Biodegredation experiments were conducted for 5 days and plasmid curing experiment was performed. Catechol test was carried out to determine phthalate degradation pathways. Results : The isolated bacteria from soil were identified as Pseudomonas putida based on 16S rRNA sequences. The size of the plasmid was estimated to be about 15.9 kb. Results of biodegradation experiments indicated that the diethyl phthalate concentrations were reduced by 85.5% after 5 days of incubation at pH 7.0 and 30 degrees C. The ability of P. putida degrading diethyl phthalate was found to be plasmid-mediated through curing experiments. [GRAPHICS] Interpretation : The study suggested that plasmid of Pseudomonas putida PAG5 could be involved in effective degradation of diethyl phthalate.
dc.description.sponsorshipMersin University Scientific Research Fund [BAP FBE BB (DC) 2012-8 YL]
dc.description.sponsorshipThis work was supported by Mersin University Scientific Research Fund (No. BAP FBE BB (DC) 2012-8 YL)
dc.identifier.doi10.22438/jeb/40/3/MRN-959
dc.identifier.endpage282
dc.identifier.issn0254-8704
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85069583056
dc.identifier.scopusqualityQ3
dc.identifier.startpage275
dc.identifier.urihttps://doi.org/10.22438/jeb/40/3/MRN-959
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21306
dc.identifier.volume40
dc.identifier.wosWOS:000464522900002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTriveni Enterprises
dc.relation.ispartofJournal of Environmental Biology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectBiodegradation
dc.subjectDiethyl phthalate
dc.subjectPhthalate esters
dc.subjectPlasmid
dc.subjectPseudomonas putida
dc.titleIsolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soil
dc.typeArticle

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