Evaluating the angiogenic and mechanical properties of hydrogels and physical constructs derived from spinal cord meninges extracellular matrix

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dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridOzudogru, Eren/0000-0002-6442-7842
dc.contributor.authorSamancioglu, Aybuke
dc.contributor.authorAydin, Beyza
dc.contributor.authorOzudogru, Eren
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2025-01-27T20:26:58Z
dc.date.available2025-01-27T20:26:58Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe vasculature is an integral unit of the tissue microenvironment due to providing nutrients and oxygen to surrounding cells. Therefore, pro-angiogenic biomaterials have the potential to improve the success of a wide range of medical therapies, including tissue engineering, wound healing, and drug delivery. Herein, we decellularized bovine spinal cord meninges with Triton X-100 and digested them with pepsin to obtain a hydrogel (MeninGEL). The cryogel form of the MeninGEL was also prepared by lyophilization process (named as MeninRIX). DNA content analysis showed that the nuclear content was significantly reduced by 98.6% after decellularization process. Furthermore, the effect of decellularization on extracellular matrix components was investigated with glycosaminoglycan (GAG) and hydroxyproline (HYP) content analyses. Tensile, compression, and suture retention tests were performed to elucidate the mechanical properties. The physiological degradation behavior of the bioscaffolds was investigated by hydrolytically. Both MeninGEL and MeninRIX have good biocompatibility and pro-angiogenic properties, as proved by the Chick Chorioallantoic Membrane (CAM) assay. Moreover, SEM and histological analyses indicated cellular migration, attachment, and dynamism on the bioscaffolds' surfaces. On the basis of these data, MeninGEL and MeninRIX are pro-angiogenic structures and have adequate mechanical properties, which makes them promising candidates for soft regenerative medicine applications.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [FLOEAP-2022-3957]
dc.description.sponsorshipWe would like to thank the Canakkale Onsekiz Mart University Science and Technology Application and Research Center (COBILTUM) staff for their technical assistance. This work was supported by Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (Project ID: FLOEAP-2022-3957). We would also like to thank Mr.Yuecel Okatali (MER-TER Medikal) for his assistance in histology studies.
dc.identifier.doi10.1088/2053-1591/acf277
dc.identifier.issn2053-1591
dc.identifier.issue8
dc.identifier.scopus2-s2.0-85169909991
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1088/2053-1591/acf277
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22523
dc.identifier.volume10
dc.identifier.wosWOS:001057779800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherIop Publishing Ltd
dc.relation.ispartofMaterials Research Express
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectdecellularization
dc.subjectspinal cord meninges
dc.subjectpro-angiogenic
dc.subjectCAM assay
dc.subjectregenerative medicine
dc.titleEvaluating the angiogenic and mechanical properties of hydrogels and physical constructs derived from spinal cord meninges extracellular matrix
dc.typeArticle

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