Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal

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dc.contributor.authorKesbic, Osman Sabri
dc.contributor.authorAcar, Umit
dc.contributor.authorKesbic, Fevziye Isil
dc.contributor.authorYılmaz, Sevdan
dc.date.accessioned2025-01-27T20:29:19Z
dc.date.available2025-01-27T20:29:19Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, and immune (TNF-alpha, IL1-ss and Il-8) and growth-related (GH and IGFI) gene expression were investigated. Five experimental diets (45% protein and 20% lipid) differed in replacement level of FM by PP at rates of 0% (control (PP0)), 25% (PP25), 50%(PP50), 75%(PP75) and 100%(PP100). Fish were fed with experimental diets in triplicate twice daily. The best growth performance was obtained in PP0 and PP25 groups. While fat ratios of fish fillets significantly differed (p < 0.05), there was no significant effects on protein ratios (p < 0.05). There was no significant change in the hematological values of fish, except those fed the PP100 diets, which displayed a reduction in eyrthocyte counts, hemoglobin content and hematocrit. As PP supplementation increased fish showed elevated serum glucose, total protein, cholesterol and myeloperoxidase activity and decreased glutamic pyruvic transaminase and alkaline phosphatase activity. Fish fed diets with between 25 and 75% replacement showed a decline in lactic acid bacteria in the gut. Significant increases in expression were observed in the liver of the PP25 fish relative to the 0% control for all immune and growth-related genes except for IL1-ss. These data suggest that up to 25% of FM can be replaced by PP without any adverse effects on rainbow trout.
dc.description.sponsorshipScientific Research Project Fund of Kastamonu University [KUE-BAP01/2018-39]
dc.description.sponsorshipAll practices on animals during the study were carried out by European Union Directive no: 2010/63 and this experiment was also approved by Kastamonu University's local ethical committee which approved this study (ref. E.10537) . Financial support for this study was provided by the Scientific Research Project Fund of Kastamonu University (Project No: KUE-BAP01/2018-39) . The authors would like to express their gratitude to Mustafa Oztuerk Akcaoglu for his assistance in enhancing the grammatical accuracy and narrative proficiency of the manuscript.
dc.identifier.doi10.1016/j.cbpb.2024.110968
dc.identifier.issn1096-4959
dc.identifier.issn1879-1107
dc.identifier.pmid38604559
dc.identifier.scopus2-s2.0-85190126560
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.cbpb.2024.110968
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22907
dc.identifier.volume273
dc.identifier.wosWOS:001240803500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofComparative Biochemistry and Physiology B-Biochemistry & Molecular Biology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectPea protein
dc.subjectRainbow trout
dc.subjectFish meal replacement
dc.subjectIntestinal microbiota
dc.subjectHealth status
dc.titleGrowth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal
dc.typeArticle

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