Structure and expression of dna methyltransferase genes from apomictic and sexual Boechera species

Basit Öğe Kaydı
dc.authoridTaşkın, Kemal Melih / 0000-0002-3746-0508
dc.authoridÖzbilen, Aslıhan / 0000-0002-2034-4682
dc.authoridSezer, Fatih / 0000-0002-9436-0191
dc.authoridHürkan, Kaan / 0000-0001-5330-7442
dc.contributor.authorTaşkın, Kemal Melih
dc.contributor.authorÖzbilen, Aslıhan
dc.contributor.authorSezer, Fatih
dc.contributor.authorHürkan, Kaan
dc.contributor.authorGüneş, Şebnem
dc.date.accessioned2025-01-27T20:14:49Z
dc.date.available2025-01-27T20:14:49Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we determined the structure of DNA methyltransferase (DNMT) genes in apomict and sexual Boechera species and investigated the expression levels during seed development. Protein and DNA sequences of diploid sexual Boechera stricta DNMT genes obtained from Phytozome 10.3 were used to identify the homologues in apomicts, Boechera holboellii and Boechera divaricaipa. Geneious R8 software was used to map the short -paired reads library of B. holboellii whole genome or B. divaricarpa transcriptome reads to the reference gene sequences. We determined three DNMT genes; for Boechera spp. METHYLTRANSFERASEI (MET I), CHROMOMETHYLASE 3 (CMT3) and DOMAINS REARRANGED METHYLTRANSFERASE 1/2 (DRM2). We examined the structure of these genes with bioinformatic tools and compared with other DNMT genes in plants. We also examined the levels of expression in silique tissues after fertilization by semi -quantitative PCR. The structure of DNMT proteins in apomict and sexual Boechera species share common features. However, the expression levels of DNMTgenes were different in apomict and sexual Boechera species. We found that DRM2 was upregulated in apomicticBoechera species after fertilization, Phylogenetic trees showed that three genes are conserved among green algae, monocotyledons and dicotyledons. Our results indicated a deregulation of DNA methylation machinery during seed development in apomicts. (C) 2016 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.compbiolchem.2016.12.002
dc.identifier.endpage21
dc.identifier.issn1476-9271
dc.identifier.issn1476-928X
dc.identifier.pmid28038368
dc.identifier.scopus2-s2.0-85007047675
dc.identifier.scopusqualityQ1
dc.identifier.startpage15
dc.identifier.urihttps://doi.org/10.1016/j.compbiolchem.2016.12.002
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21208
dc.identifier.volume67
dc.identifier.wosWOS:000395598000003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofComputational Biology and Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDNA methylation
dc.subjectDNA methyltransferases
dc.subjectBoechera
dc.subjectApomixis
dc.subjectSeed development
dc.titleStructure and expression of dna methyltransferase genes from apomictic and sexual Boechera species
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu