In-vitro and In-silico evaluation of the inhibitory effects of Persea americana leaf extract against calcium oxalate stones

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dc.contributor.authorAkyol, Emel
dc.contributor.authorDanisman, Merve
dc.contributor.authorOner, Mualla
dc.date.accessioned2026-02-03T12:03:07Z
dc.date.available2026-02-03T12:03:07Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study investigated the effects of Persea americana extract (PAE) as a potential inhibitor of calcium oxalate monohydrate growth using both in-vitro and in-silico methods with spontaneous crystallization to evaluate its effectiveness in preventing kidney stones. X-ray diffraction was used to characterize the morphology of calcium oxalate (CaOx) crystals, while scanning electron microscopy (SEM) was used to determine their particle size and crystal formation patterns. SwissADME (Absorption, Distribution, Metabolism, Excretion) computational analysis predicted the biological activity of the extract. The experimental results show that the inhibition of crystal growth increases with the amount of PAE in solution, and crystal growth was almost completely inhibited for 450 min at a concentration of 100 mL of PAE. The in vitro results also revealed that Persea americana leaf promoted the formation of calcium oxalate dihydrate (COD) crystals rather than monohydrate crystals. These findings on PAE's inhibitory effect on calcium oxalate crystallization suggest that natural additives could be valuable in treating kidney stone disease.
dc.description.sponsorshipYimath;ldimath;z Technical University [2012-07-01-YL05]
dc.description.sponsorshipResearch Fund of the Yildiz Technical University
dc.description.sponsorshipThis work was supported by the Research Fund of the Yildiz Technical University. Project Number 2012-07-01-YL05. Here, we thank to the new SwissADME web tool that gives free access to a pool of fast yet robust predictive models for physicochemical properties, pharmacokinetics, drug-likeness and medicinal chemistry friendliness.
dc.identifier.doi10.1007/s00240-025-01791-4
dc.identifier.issn2194-7228
dc.identifier.issn2194-7236
dc.identifier.issue1
dc.identifier.pmid40586908
dc.identifier.scopus2-s2.0-105010108328
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s00240-025-01791-4
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34977
dc.identifier.volume53
dc.identifier.wosWOS:001520314800004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofUrolithiasis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectPersea americana extract (PAE)
dc.subjectRenal calculi
dc.subjectSwissADME
dc.subjectInhibitor
dc.subjectCalcium oxalate crystallization
dc.titleIn-vitro and In-silico evaluation of the inhibitory effects of Persea americana leaf extract against calcium oxalate stones
dc.typeArticle

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