Salinity response of transgenic potato genotypes expressing the oxalate oxidase gene

dc.contributor.authorTurhan, Hakan
dc.date.accessioned2025-01-27T19:00:25Z
dc.date.available2025-01-27T19:00:25Z
dc.date.issued2005
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn vitro and in vivo responses of transgenic and non-transgenic potato (Solanum tuberosum L.) genotypes to salinity were investigated. A gene responsible for the production of oxalate oxidase enzyme, whose expression has been associated with plant stress tolerance, was used. Transgenic potato plants expressing the oxalate oxidase enzyme were produced using Agrobacterium-mediated transformation. The transgenic nature of the plants was verified by their antibiotic (kanamycin) resistance and then in vitro enzyme assays. Four independent transgenic genotypes obtained from 2 cultivars, Maris Bard and Desiree, were tested at 5 levels of NaCl (0%, 0.25%, 0.50%, 0.75% and 1.00% w/v) in vitro. The same genotypes were also tested at 3 levels of NaCl (0%, 0.50% and 0.75% w/v) under glasshouse conditions (in vivo). The results revealed that the growth of all genotypes was significantly inhibited by increasing salt treatments under both in vitro and in vivo conditions. Transgenic genotypes showed a relatively higher salinity tolerance than the non-transgenic genotypes in vitro. On the other hand, in the glasshouse, the results were less consistent but some transgenic genotypes showed superior tuber yield characteristics to the non-transgenics under salt stress. © Tübi?tak.
dc.identifier.doi10.3906/tar-0401-11
dc.identifier.endpage195
dc.identifier.issn1300-011X
dc.identifier.issue3
dc.identifier.scopus2-s2.0-18944395892
dc.identifier.scopusqualityQ1
dc.identifier.startpage187
dc.identifier.trdizinid44657
dc.identifier.urihttps://doi.org/10.3906/tar-0401-11
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/44657
dc.identifier.urihttps://hdl.handle.net/20.500.12428/13316
dc.identifier.volume29
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250125
dc.subjectEnzyme; Oxalate oxidase; Salinity; Solanum tuberosum
dc.titleSalinity response of transgenic potato genotypes expressing the oxalate oxidase gene
dc.typeArticle

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