Determination of the expression levels of DNA methyltransferase genes during a highly efficient regeneration system via shoot organogenesis in the diploid apomict Boechera divaricarpa

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dc.authoridSezer, Fatih / 0000-0002-9436-0191
dc.authoridÖzbilen, Aslıhan / 0000-0002-2034-4682
dc.authoridTaşkın, Kemal Melih / 0000-0002-3746-0508
dc.contributor.authorTaşkın, Kemal Melih
dc.contributor.authorÖzbilen, Aslıhan
dc.contributor.authorSezer, Fatih
dc.contributor.authorCorduk, Nurşen
dc.contributor.authorErden, Damla
dc.date.accessioned2025-01-27T20:59:45Z
dc.date.available2025-01-27T20:59:45Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we established the first efficient method to regenerate diploid apomict Boechera divaricarpa via shoot organogenesis. Hypocotyl explants were cultured on MS medium supplemented with plant growth regulators. The morphogenic potential of B. divaricarpa hypocotyl tissue was investigated to establish an efficient adventitious shoot regeneration system. We tested hypocotyls from 7-day-old in vitro seedlings. The effect of various concentrations of cytokinin and auxin on in vitro regeneration of these explants was also investigated. We found that callus induction and shoot regeneration were significantly affected by the concentrations and types of plant growth regulators. MS medium supplemented with 17.75 mu M benzylaminopurine and 0.53 mu M naphthaleneacetic acid gave the highest number of shoots per explant after 4 weeks in culture. We also determined the expression levels of three DNA methyltransferase genes under tissue culture conditions to understand their activities during callus induction and shoot regeneration. BdMET1 was expressed at low levels during both callus induction and shoot regeneration, while expression of BdDRM2 was high during callus induction and low during shoot regeneration. BdCMT3 was highly expressed in both hypocotyl explants and seedlings and may be a good candidate to induce epigenetic variations in diploid apomict B. divaricarpa shoots derived from tissue culture. Our study provides an efficient in vitro regeneration method and determines the expression levels during tissue culture of methyltransferase genes; it can be used as a new tool to understand the molecular biology of apomixis in B. divaricarpa.
dc.identifier.doi10.1007/s11240-014-0704-0
dc.identifier.endpage343
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84939935909
dc.identifier.scopusqualityQ1
dc.identifier.startpage335
dc.identifier.urihttps://doi.org/10.1007/s11240-014-0704-0
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26816
dc.identifier.volume121
dc.identifier.wosWOS:000352652800007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPlant Cell Tissue and Organ Culture
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBoechera divaricarpa
dc.subjectIn vitro
dc.subjectRegeneration
dc.subjectTissue culture
dc.subjectAdventitious shoot
dc.subjectDNA methyltransferases
dc.titleDetermination of the expression levels of DNA methyltransferase genes during a highly efficient regeneration system via shoot organogenesis in the diploid apomict Boechera divaricarpa
dc.typeArticle

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