Biodegradation of 2,4-dinitrotoluene (DNT) by Arthrobacter sp K1 isolated from a crude oil contaminated soil

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dc.authoridKantar, Cetin/0000-0001-9747-9115
dc.authoridCoral, Gokhan/0000-0002-3533-118X
dc.contributor.authorKuce, Pinar
dc.contributor.authorCoral, Gokhan
dc.contributor.authorKantar, Cetin
dc.date.accessioned2025-01-27T20:39:05Z
dc.date.available2025-01-27T20:39:05Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractLaboratory experiments were performed to characterize and identify 2,4-dinitrotoluene (DNT)-degrading bacterial strains isolated from crude oil contaminated soil from a landfill dump site of a petroleum refinery in Mersin, Turkey. Fluorescent in situ hybridization (FISH) with dntAa probes encoding 2,4-DNT dioxygenase was used to detect 2,4-DNT-degrading bacteria. The direct FISH analysis of soil samples collected from a petroleum refinery showed very weak signals. Therefore, a selective enrichment culture technique using 2,4-DNT as the sole carbon source was then used to isolate DNT degrading bacteria. Following the culture enrichment procedure, the hybridization signals improved significantly in the isolated bacterial strains. Based on 16S rRNA sequences, the bacteria isolated from the soil samples were identified as Arthrobacter sp. Results from the batch biodegradation experiments indicate that the biodegradation rates of 2,4-DNT with this strain were highly dependent on environmental conditions such as pH and temperature, with optimum conditions obtained at 30 A degrees C and pH similar to 7. A first-order kinetic model was able to accurately describe 2,4-DNT degradation rates under different environmental conditions (e.g., pH). The ability of Arthrobacter sp. for degrading 2,4-DNT was found to be plasmid-mediated through curing experiments. The size of the plasmid involved, referred to as pArK1, was estimated to be about 8.1 kb.
dc.description.sponsorshipMersin University [2008-8YL]
dc.description.sponsorshipThis work has been supported by Mersin University Research Fund (Project Code: BAP-FBE BB (PK) 2008-8YL.
dc.identifier.doi10.1007/s13213-014-0880-5
dc.identifier.endpage476
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84897365502
dc.identifier.scopusqualityQ2
dc.identifier.startpage467
dc.identifier.urihttps://doi.org/10.1007/s13213-014-0880-5
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23856
dc.identifier.volume65
dc.identifier.wosWOS:000350219500045
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherBMC
dc.relation.ispartofAnnals of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectBiodegradation
dc.subjectArthrobacter
dc.subject2,4-Dinitrotoluene (DNT)
dc.subjectPlasmid
dc.subjectpArK1
dc.subjectFluorescence in situ hybridization (FISH)
dc.titleBiodegradation of 2,4-dinitrotoluene (DNT) by Arthrobacter sp K1 isolated from a crude oil contaminated soil
dc.typeArticle

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