MerR-fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking water

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dc.authoridEvran, Serap/0000-0001-6676-4888
dc.contributor.authorOzyurt, Canan
dc.contributor.authorUstukarci, Handan
dc.contributor.authorEvran, Serap
dc.contributor.authorTelefoncu, Azmi
dc.date.accessioned2025-01-27T20:59:40Z
dc.date.available2025-01-27T20:59:40Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMercury ion (Hg2+) is a universal pollutant and its detection is crucial for public healthcare. In this study, we developed a novel fluorescent biosensor by construction of a protein fusion between the mercury-sensing transcription factor MerR and enhanced yellow fluorescent protein (EYFP). Hg2+-induced conformational change of MerR was transduced into fluorescence signal. Fluorescence intensity of the biosensor protein decreased with increasing concentrations of Hg2+ and a linear response was obtained in the range of 0.5-40 nM. The limit of detection was 0.5 nM, which was much lower than the maximum allowed level in water. The biosensor specificity was highly dependent on type and concentration of metal ion. The biosensor exhibited high specificity in a mixture of metal ions at 0.5 nM concentration. However, the interference effect was more pronounced at 40 nM concentration of metal ions. The measurement was completed in less than 1 Min with no need for sample preparation or preincubation steps. The biosensor achieved accurate and reliable detection in the spiked drinking water sample, as validated by the inductively coupled plasma optical emission spectrometry.
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [2011 FEN 035] Funding Source: Medline
dc.identifier.doi10.1002/bab.1805
dc.identifier.endpage737
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue5
dc.identifier.pmid31411759
dc.identifier.scopus2-s2.0-85071356618
dc.identifier.scopusqualityQ2
dc.identifier.startpage731
dc.identifier.urihttps://doi.org/10.1002/bab.1805
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26777
dc.identifier.volume66
dc.identifier.wosWOS:000491221600003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHg2+
dc.subjectfluorescent protein
dc.subjectmercury biosensor
dc.titleMerR-fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking water
dc.typeArticle

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