Genotoxic and cytotoxic effects of polystyrene nanoplastics on human lymphocytes: A comprehensive analysis

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dc.contributor.authorBerber, Ahmet Ali
dc.contributor.authorKenanoglu, Nihan Akinci
dc.contributor.authorDemir, Sefika Nur
dc.contributor.authorAksoy, Huseyin
dc.date.accessioned2025-05-29T02:57:58Z
dc.date.available2025-05-29T02:57:58Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA growing amount of plastic waste is finding its way into natural ecosystems as a result of the widespread usage of plastics in modern society. These wastes degrade physically and biologically over time, transforming into microplastics (MPs) and nanoplastics (NPs). MPs and NPs emissions from the terrestrial environment then mix with rivers and eventually the seas, forming garbage. The cytotoxic and genotoxic effects of 50 nm polystyrene nanoplastics (PsNP) on human lymphocytes were assessed using the in vitro mitotic index (MI), micronucleus (MN), and comet assays. Both 24 and 48-h applications were performed for MI, and it was determined that 50 nm PsNP provided a statistically significant decrease in MI compared to the control at all concentrations and application times (except 0.001 and 0.1 mu g/mL at 24 h). According to the MN test results, the MN frequency increased significantly at all concentrations when compared to the negative control. In the comet test, a statistically significant increase of comet tail length was observed at 0.001, 10 and 100 mu g/mL concentration with 50 nm PsNP exposure. Tail moment also showed a statistically significant increase at the lowest concentration of 0.001 mu g/mL and the highest concentration of 1, 10, 100 mu g/mL compared to the negative control. All test results show that PsNP has both genotoxic and cytotoxic potential.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [FBA-2020-3162]
dc.description.sponsorshipWe thank to Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (FBA-2020-3162) .
dc.identifier.doi10.1016/j.mrgentox.2025.503850
dc.identifier.issn1383-5718
dc.identifier.issn1879-3592
dc.identifier.pmid40044373
dc.identifier.scopus2-s2.0-85215993420
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.mrgentox.2025.503850
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30214
dc.identifier.volume902
dc.identifier.wosWOS:001413480600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMutation Research-Genetic Toxicology and Environmental Mutagenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250529
dc.subjectNanoplastic
dc.subjectToxicity
dc.subjectComet assay
dc.subjectMicronucleus
dc.subjectHuman lymphocyte
dc.titleGenotoxic and cytotoxic effects of polystyrene nanoplastics on human lymphocytes: A comprehensive analysis
dc.typeArticle

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