A molecular assay for quantification and simultaneous detection of soybean and poultry DNA in sausages following multi-extraction

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dc.contributor.authorSakalar, Ergun
dc.date.accessioned2025-01-27T20:43:44Z
dc.date.available2025-01-27T20:43:44Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA duplex PCR assay was designed for simultaneous identification of soybean and poultry in sausages. A Multi-Fast DNA Isolation Kit was used for simultaneous extraction of amplifiable soybean and poultry DNA. Primers generated specific DNA fragments of 173 and 183 bp in length for soybean and poultry, respectively. The duplex assay sensitivity was 0.35% for specific detection of poultry mixed with soybeans and other additive materials and was 0.05% for specific detection of soybeans mixed with poultry and other additive materials. The optimized duplex PCR assay was applied to 15 commercial sausages. Unintentional and intentional contaminants were analyzed using real-time PCR based Sakalar Quantification Table of DNA (SQT-DNA). Techniques developed here are suitable tools for qualitative and quantitative comparison of sausage contents.
dc.identifier.doi10.1007/s10068-015-0252-3
dc.identifier.endpage1920
dc.identifier.issn1226-7708
dc.identifier.issn2092-6456
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84948958290
dc.identifier.scopusqualityQ2
dc.identifier.startpage1915
dc.identifier.urihttps://doi.org/10.1007/s10068-015-0252-3
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24355
dc.identifier.volume24
dc.identifier.wosWOS:000365766300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherKorean Society Food Science & Technology-Kosfost
dc.relation.ispartofFood Science and Biotechnology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectPCR
dc.subjectduplex
dc.subjectSQT-DNA
dc.subjectmeat
dc.subjectfood
dc.titleA molecular assay for quantification and simultaneous detection of soybean and poultry DNA in sausages following multi-extraction
dc.typeArticle

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