A Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications

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dc.authoridSezgin Arslan, Tugba/0000-0003-2547-6120
dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.contributor.authorDemiray, Elif Beyza
dc.contributor.authorSezgin Arslan, Tugba
dc.contributor.authorDerkus, Burak
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2025-01-27T20:22:25Z
dc.date.available2025-01-27T20:22:25Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, it is aimed to fabricate a novel silk sericin (SS)/wool keratin (WK) hydrogel-based scaffolds using an in situ bubble-forming strategy containing an N-(3-dimethylaminopropyl)-N '-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling reaction. During the rapid gelation process, CO2 bubbles are released by activating the carboxyl groups in sericin with EDC and NHS, entrapped within the gel, creating a porous cross-linked structure. With this approach, five different hydrogels (S2K1, S4K2, S2K4, S6K3, and S3K6) are constructed to investigate the impact of varying sericin and keratin ratios. Analyses reveal that more sericin in the proteinaceous mixture reinforced the hydrogel network. Additionally, the hydrogels' pore size distribution, swelling ratio, wettability, and in vitro biodegradation rate, which are crucial for the applications of biomaterials, are evaluated. Moreover, biocompatibility and proangiogenic properties are analyzed using an in-ovo chorioallantoic membrane assay. The findings suggest that the S4K2 hydrogel exhibited the most promising characteristics, featuring an adequately flexible and highly porous structure. The results obtained by in vitro assessments demonstrate the potential of S4K2 hydrogel in muscle tissue engineering. However, further work is necessary to improve hydrogels with an aligned structure to meet the features that can fully replace muscle tissue for volumetric muscle loss regeneration. A novel hydrogel-based bioengineered scaffold with a porous and flexible ultrastructure is fabricated via in situ crosslinking of sericin and keratin. In chorioallantoic membrane analysis, the bioengineered scaffold not only shows angiogenic potential but also promotes the biological behavior of C2C12 muscle cells. These results highlight the potential of the sericin/keratin scaffold for future applications in repairing volumetric muscle tissue loss. image
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [FYL-2023-4479]
dc.description.sponsorshipThe authors thanked the Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (Project ID: FYL-2023-4479) for the financial support and acknowledge the Canakkale Onsekiz Mart University Science and Technology Application and Research Center (COBILTUM) providing facilities for analysis. The authors also thanked Mr. Yuecel Okatali (MER-TER Medical, Eski & scedil;ehir, Tuerkiye) for the sectioning and HC staining of the tissues.
dc.identifier.doi10.1002/mabi.202400362
dc.identifier.issn1616-5187
dc.identifier.issn1616-5195
dc.identifier.pmid39427341
dc.identifier.scopus2-s2.0-85206602759
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mabi.202400362
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21873
dc.identifier.wosWOS:001336144400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjecthydrolyzed wool-keratin
dc.subjectin situ bubble-forming hydrogel entrapment
dc.subjectmuscle tissue engineering
dc.subjectmyogenesis
dc.subjectwater-extracted silk sericin
dc.titleA Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications
dc.typeArticle

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