Inhibition of browning problem during micropropagation of Sideritis trojana bornm., an endemic medicinal herb of Turkey

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dc.contributor.authorCorduk, Nursen
dc.contributor.authorAkı, Cüneyt
dc.date.accessioned2025-01-27T21:02:06Z
dc.date.available2025-01-27T21:02:06Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMicropropagation is used increasingly for ex situ conservation of endangered endemic plants and this in vitro technique provides mass propagation of medicinal plants. However during this process, stages of micropropagation are limited by serious problems. One of these problems is the explant browning which is related to the oxidation of phenolic compounds. In this research, several different methods have been used to eliminate the browning problem in tissue culture. In the present research, inhibitory effects of different treatments such as Heller medium or adding activated charcoal, morpholine ethane sulfonic acid (MES) or ascorbic acid/citric acid combination to the medium, fast subculture passages or changing culture conditions to the dark were investigated against browning problem of Sideritis trojana Bornm. Adding a combination of 100 mg/l ascorbic acid and 50 mg/l citric acid to the Murashige and Skoog (MS) medium was found as the most effective treatment.
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey [107T125]
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey (Project No: 107T125).
dc.identifier.endpage6765
dc.identifier.issn1224-5984
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84858311274
dc.identifier.scopusqualityN/A
dc.identifier.startpage6760
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27280
dc.identifier.volume16
dc.identifier.wosWOS:000299089600010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherArs Docendi
dc.relation.ispartofRomanian Biotechnological Letters
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectSideritis trojana
dc.subjectbrowning problem
dc.subjectphenolic compounds
dc.subjectmicropropagation
dc.titleInhibition of browning problem during micropropagation of Sideritis trojana bornm., an endemic medicinal herb of Turkey
dc.typeArticle

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