Amperometric sensor for total antioxidant capacity measurement using Cu (II)-neocuproine/carrageenan-MWCNT/GCE

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dc.authoridBener, Mustafa/0000-0002-2699-1354
dc.authoridSen, Furkan Burak/0000-0001-6857-392X
dc.authorid, Ervanur Elmas/0009-0007-5528-7044
dc.contributor.authorSen, Furkan Burak
dc.contributor.authorElmas, Ervanur
dc.contributor.authorDilgin, Yusuf
dc.contributor.authorBener, Mustafa
dc.contributor.authorApak, Resat
dc.date.accessioned2025-01-27T20:22:27Z
dc.date.available2025-01-27T20:22:27Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractDetermination of total antioxidant capacity (TAC) in foods is very important for combating oxidative stressinduced diseases. For this purpose, an amperometric sensor was developed for TAC determination by manufacturing a new electrode consisting of a copper(II)-neocuproine (Cu(II)-Nc) and carrageenan-multi-walled nanotube suspension functionalized on a glassy carbon electrode, named as Cu(II)-Nc/Car-MWCNT/GCE. Cyclic voltammetric studies showed that the modified electrode exhibits a very well-formed reversible redox couple for the Cu(II)-Nc/Cu(I)-Nc complex. For amperometric sensor preparation, the Cu(II)-Nc cationic chelate was electrostatically retained on the electrode with the anionic groups of Car (a sulfated biopolymer of low cost) and immobilized on the surface. Cu(II)-Nc was reduced to Cu(I)-Nc on the electrode with the addition of antioxidants under constant potential, thereby functioning as an electron-transfer mediator. Thus, the individual antioxidant compounds were not measured at their own peak potentials but were indirectly measured at a common potential through their ability to reduce cupric-to-cuprous neocuproine. The reoxidation current of Cu(I)-Nc showing a proportional increase with antioxidant concentration was recorded, resulting in increased sensitivity and selectivity. This anodic current intensity of Cu(I)-Nc correlated with the total antioxidant capacity (TAC) of real samples such as plant extracts, reflecting the combined reducing ability of all antioxidants in the sample. Calibration graphs of antioxidants were obtained by optimizing the working conditions. The current intensity of Cu (I)-Nc arising from trolox varied linearly with concentration in the range of 4.98-84.39 mu M. The limit of detection and limit of quantification values for trolox were found to be 0.59 and 1.99 mu M, respectively. The developed method selectively responded to food antioxidants, and was not affected by potential interferent ions and molecules commonly found in foods. The TAC values of real samples such as herbal teas, plant extracts, and fruit juices were calculated and compared with those found by the conventional spectrophotometric CUPRAC method.
dc.description.sponsorshipTUBITAK (Turkish Scientific and Technical Research Council); National Leading Researchers Program Project [120C134]; Istanbul University Research Fund; Bilimsel Arastirma Projeleri (BAP) Yurutucu Sekreterligi; Turkish Academy of Sciences (TUBA), TUBA Outstanding Young Scientist Award Programme (GEBIP)
dc.description.sponsorshipResat Apak expresses his gratitude to TUBITAK (Turkish Scientific and Technical Research Council) for the 2247-A National Leading Researchers Program Project (Project No: 120C134). Mustafa Bener expresses his gratitude to the Istanbul University Research Fund, Bilimsel Arastirma Projeleri (BAP) Yurutucu Sekreterligi (Project No: FBG-2022-38594). Mustafa Bener was supported by the Turkish Academy of Sciences (TUBA) in part by the TUBA Outstanding Young Scientist Award Programme (GEBIP).
dc.identifier.doi10.1016/j.microc.2024.110081
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85184580672
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2024.110081
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21891
dc.identifier.volume199
dc.identifier.wosWOS:001185057700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectElectrochemical CUPRAC sensor
dc.subjectTotal antioxidant capacity
dc.subjectCarrageenan biopolymer
dc.subjectMultiwalled carbon nanotubes
dc.titleAmperometric sensor for total antioxidant capacity measurement using Cu (II)-neocuproine/carrageenan-MWCNT/GCE
dc.typeArticle

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