Improved drought tolerance of EMS mutagenized Alfalfa (Medicago sativa L.) mutants by in vitro screening at germination stage

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dc.authorid/0000-0001-7564-997X
dc.authoridTiryaki, iskender/0000-0002-7504-2892
dc.contributor.authorTiryaki, Iskender
dc.contributor.authorSari, Ugur
dc.contributor.authorCetin, Selcuk
dc.contributor.authorAcar, Okan
dc.date.accessioned2025-01-27T21:01:28Z
dc.date.available2025-01-27T21:01:28Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe objectives of this study were to determine drought tolerant novel mutant of alfalfa (Medicago sativa L.) genotypes by screening EMS mutagenized 340675 M-3 seeds at germination stages in the presence of osmotic stress of 35% PEG(6000). Root growth assay provided several drought tolerant candidate mutants. Of those, 4 mutants were further evaluated at water deficit conditions applied for 24 days after the first cutting at flowering bud stage. The results revealed that mutants determined as drought tolerant at germination stage were also tolerant to water deficit conditions. Protein content and superoxide dismutase values were found to be higher in all mutants than controls. Ascorbate peroxides, glutton reductase and lipid peroxidase values varied based on the mutant genotype and duration of drought stress. Drought stress significantly changed transcriptional levels of MtP5CS, MtDehyd, MseIF-2, MtRD2 and MsNAC genes. These results indicated that in vitro screening of alfalfa mutant seeds for osmatic tolerance at germination and early seedling growth stages was successfully able to determine the drought tolerant alfalfa mutants which were also tolerant to water deficit conditions after the first cutting at flowering bud stage.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG-116O417]
dc.description.sponsorshipThe financial support of this study was provided by Scientific and Technological Research Council of Turkey (TUBITAK) with research grant TOVAG-116O417. We would like to acknowledge Dr. Melih Taskin who allowed us to use RT-qPCR. We would also like to thank Huseyin Keles and Semun Tayyar for their help on harvesting and selfing in the field, respectively. We also would like to acknowledge Enes Gokhan Yilmaz, Beste Celep, Erman Cavusoglu and anonymous lab members who helped during the laboratory experiments and mutant growth in control conditions.
dc.identifier.doi10.1038/s41598-022-16294-0
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid35882960
dc.identifier.scopus2-s2.0-85134816616
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-022-16294-0
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27046
dc.identifier.volume12
dc.identifier.wosWOS:000835004100037
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectSuperoxide-Dismutase
dc.subjectStress
dc.subjectAscorbate
dc.subjectEvolution
dc.subjectSalt
dc.titleImproved drought tolerance of EMS mutagenized Alfalfa (Medicago sativa L.) mutants by in vitro screening at germination stage
dc.typeArticle

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