Determination of Pathogenicity of Bacteria Isolated from Some Aquatic Amphibian and Reptile Species

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dc.authoridKARAKAS, ILKE/0000-0001-6596-0879
dc.contributor.authorKarakas, I.
dc.contributor.authorDogru, N. Hacioglu
dc.contributor.authorGul, C.
dc.contributor.authorTosunoglu, M.
dc.date.accessioned2025-01-27T20:35:01Z
dc.date.available2025-01-27T20:35:01Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMicroflora of reptiles and amphibians pose a serious threat to the environment and humans, as these bacteria are more resistant to antimicrobials or have more virulence factors. In this study, we focused on the characterization of Gram-negative bacterial isolates obtained from Mauremys rivulata (Western Caspian turtle), Natrix natrix (Grass snake), Natrix tessellata (Dice snake) and Pelophylax bedriagae (Levantine frog) using cultural methods and determine some virulence factors (antibiotic resistance, VanA and TetM resistant genes, biofilm, siderophore and some enzymatic activities etc.). Multiple antibiotic resistance (MAR) and virulence index (VI) of each strain were also calculated. A total of 85 Gram-negative isolates exhibited varying levels of antibiotic resistance and the ability to produce one or more extracellular enzymes such as amylase, lecithinase, protease, lipase, hemolysis, siderophore, amino acid decarboxylase and DNase. Although the TetM has been identified in all 7 isolates, none of the isolates have shown the presence of the VanA gene. According to MAR and VI results, most of the isolates were found to be in the high threat group and these indexes were correlated with antibiotic resistance. The study data revealed that chemicals used in industry and agriculture both trigger environmental pollution and pose risk factors for wildlife.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113Z098]
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK Project no. 113Z098). We are grateful to TUBITAK.
dc.identifier.doi10.1134/S1062359024604725
dc.identifier.endpage872
dc.identifier.issn1062-3590
dc.identifier.issn1608-3059
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85195134748
dc.identifier.scopusqualityQ4
dc.identifier.startpage857
dc.identifier.urihttps://doi.org/10.1134/S1062359024604725
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23547
dc.identifier.volume51
dc.identifier.wosWOS:001236820200017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPleiades Publishing Inc
dc.relation.ispartofBiology Bulletin
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTetM-VanA resistance gene
dc.subjectsiderophore
dc.subjectantibiotic resistant
dc.subjectbiofilm
dc.subjectvirulence index
dc.titleDetermination of Pathogenicity of Bacteria Isolated from Some Aquatic Amphibian and Reptile Species
dc.typeArticle

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