Trans-differentiation of human adipose-derived mesenchymal stem cells into cardiomyocyte-like cells on decellularized bovine myocardial extracellular matrix-based films

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dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorGalata, Yusuf Furkan
dc.contributor.authorArslan, Tugba Sezgin
dc.contributor.authorDerkus, Burak
dc.date.accessioned2025-01-27T20:16:36Z
dc.date.available2025-01-27T20:16:36Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we aimed at fabricating decellularized bovine myocardial extracellular matrix-based films (dMEbF) for cardiac tissue engineering (CTE). The decellularization process was carried out utilizing four consecutive stages including hypotonic treatment, detergent treatment, enzymatic digestion and decontamination, respectively. In order to fabricate the dMEbF, dBM were digested with pepsin and gelation process was conducted. dMEbF were then crosslinked with N-hydroxysuccinimide/1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (NHS/EDC) to increase their durability. Nuclear contents of native BM and decellularized BM (dBM) tissues were determined with DNA content analysis and agarose-gel electrophoresis. Cell viability on dMEbF for 3rd, 7th, and 14th days was assessed by MTT assay. Cell attachment on dMEbF was also studied by scanning electron microscopy. Trans-differentiation capacity of human adipose-derived mesenchymal stem cells (hAMSCs) into cardiomyocyte-like cells on dMEbF were also evaluated by histochemical and immunohistochemical analyses. DNA contents for native and dBM were, respectively, found as 886.11 +/- 164.85 and 47.66 +/- 0.09 ng/mg dry weight, indicating a successful decellularization process. The results of glycosaminoglycan and hydroxyproline assay, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), performed in order to characterize the extracellular matrix (ECM) composition of native and dBM tissue, showed that the BM matrix was not damaged during the proposed method. Lastly, regarding the histological study, dMEbF not only mimics native ECM, but also induces the stem cells into cardiomyocyte-like cells phenotype which brings it the potential of use in CTE.
dc.description.sponsorshipMinistry of Science, Industry and Technology, Republic of Turkey [0089.TGSD.2013]; Scientific and Technological Research Council of Turkey [114S851]
dc.description.sponsorshipWork on this paper was financially supported by Ministry of Science, Industry and Technology, Republic of Turkey (Project ID. 0089.TGSD.2013) and the Scientific and Technological Research Council of Turkey (Project ID. 114S851). We wish to thank Canakkale Onsekiz Mart University, Science and Technology Application & Research Center and MER-TER Medical for collaborating with analyses.
dc.identifier.doi10.1007/s10856-018-6135-4
dc.identifier.issn0957-4530
dc.identifier.issn1573-4838
dc.identifier.issue8
dc.identifier.pmid30056552
dc.identifier.scopus2-s2.0-85051145203
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s10856-018-6135-4
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21313
dc.identifier.volume29
dc.identifier.wosWOS:000440181000003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Materials Science-Materials in Medicine
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiologic Scaffold
dc.subjectFabrication
dc.subjectHydrogels
dc.subjectCollagen
dc.subjectTherapy
dc.subjectSupport
dc.subjectTissues
dc.titleTrans-differentiation of human adipose-derived mesenchymal stem cells into cardiomyocyte-like cells on decellularized bovine myocardial extracellular matrix-based films
dc.typeArticle

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