Fluorimetric determination of Ascorbic Acid in Vitamin C tablets using methylene blue

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.contributor.authorDilgin, Y
dc.contributor.authorNisli, G
dc.date.accessioned2025-01-27T20:12:19Z
dc.date.available2025-01-27T20:12:19Z
dc.date.issued2005
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, a simple and sensitive fluorimetric method was described for the determination of Ascorbic Acid (AA). The procedure is based on the reaction between AA and Methylene Blue (MB). The fluorescence intensity of NIB was measured at excitation and emission of 664 and 682 nm, respectively. MB concentration was decreased as a function of decreasing fluorescence intensity due to forming colorless form of MB (Leuco-MB) in the reaction between AA and NIB. A linear relationship was obtained between the decreasing fluorescence intensity and the concentration of AA in the range of 3.0x10(-7)-6.0x10(-6) mol(.)l(-1). The detection limit was 2.5x10(-7) mol(.)l(-1). The proposed method was applied successfully for the determination of AA in Vitamin C tablets.
dc.identifier.doi10.1248/cpb.53.1251
dc.identifier.endpage1254
dc.identifier.issn0009-2363
dc.identifier.issue10
dc.identifier.pmid16204979
dc.identifier.scopus2-s2.0-26444531608
dc.identifier.scopusqualityQ3
dc.identifier.startpage1251
dc.identifier.urihttps://doi.org/10.1248/cpb.53.1251
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20910
dc.identifier.volume53
dc.identifier.wosWOS:000232679900007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPharmaceutical Soc Japan
dc.relation.ispartofChemical & Pharmaceutical Bulletin
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectfluorimetric determination
dc.subjectAscorbic Acid
dc.subjectmethylene blue
dc.subjectVitamin C tablets
dc.titleFluorimetric determination of Ascorbic Acid in Vitamin C tablets using methylene blue
dc.typeArticle

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