A novel electrochemical immunosensor based on disposable ITO-PET electrodes for sensitive detection of PAK 2 antigen

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dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:49:48Z
dc.date.available2025-01-27T20:49:48Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present work, we have developed a novel electrochemical immunosensor based on modified disposable ITO-PET (indium tin oxide - polyethylene terephthalate) electrodes for PAK-2 (p21-activated kinase 2) early detection. An ITO-PET electrode surface was modified with 3-cyanopropyl trimethoxysilane (3-CPTMS), which formed self-assembled monolayers (SAMs). PAK2 immunosensing was performed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and a single frequency impedance (SFI) technique utilized for specific interaction between the anti-PAK2 and PAK2 antigen. Further, the morphological characterization of each ITO electrode surface was observed by scanning electron microscopy (SEM). All parameters such as 3-CPTMS concentration, antibody concentration and antibody incubation time were optimized. Analytical characteristics of the proposed immunosensor such as linear determination range, repeatability, reproducibility, regeneration, storage life and surface coverage of immunosensors were determined. The PAK2 electrochemical immunosensor performed flawlessly with a wide determination range (from 0.05 pg mL(-1) to 2.5 pg mL(-1)) and low limits of detection (0.0252 pg mL(-1)) and of quantification (0.0842 pg mL(-1)) The artificial and human serum samples were tested to verify the viability of the proposed immunosensor. (C) 2019 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113 Z 678]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, Project number: 113 Z 678).
dc.identifier.doi10.1016/j.jelechem.2019.113304
dc.identifier.issn1572-6657
dc.identifier.issn1873-2569
dc.identifier.scopus2-s2.0-85069678809
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jelechem.2019.113304
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25317
dc.identifier.volume848
dc.identifier.wosWOS:000504505400050
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofJournal of Electroanalytical Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectITO-PET
dc.subjectPAK-2
dc.subjectBiosensor
dc.subjectSingle frequency impedance (SFI) technique
dc.titleA novel electrochemical immunosensor based on disposable ITO-PET electrodes for sensitive detection of PAK 2 antigen
dc.typeArticle

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