Investigation of biological properties, double-stranded RNA patterns and antigen concentration in citrus species infected with citrus tristeza virus

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dc.contributor.authorKorkmaz, S
dc.date.accessioned2025-01-27T20:14:22Z
dc.date.available2025-01-27T20:14:22Z
dc.date.issued2002
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBiological properties and dsRNA patterns of one Cyprus and three Turkish isolates of citrus tristeza virus (CTV) were investigated. In addition, CTV antigen concentration and effect of tissue sampling time from naturally infected Shamouti sweet orange trees grown in the field of Icel Province, Turkey, were also determined. The Cyprus isolate showed vein clearing symptoms on grapefruit, 'Madam Vinous' and Mexican lime and stem pitting symptoms on Mexican lime. The three Turkish isolates showed only vein clearing symptoms on Mexican lime. All four isolates showed a full-length major double-stranded RNA (dsRNA) band of 13.3 x 10(6) Da mol.wt in extracts from infected Madam Vinous sweet orange trees, and major or minor dsRNA bands with 2.0. 0.8 and 0.5 x 10(6) mol.wt. All seven different citrus varieties inoculated with the Igdir (D) strain contained full-length dsRNA. The additional two dsRNA of 0.8 and 0.5 x 10(6) mol.wt were also detected as clearly as full-length dsRNA in these hosts, but were weaker in Citrus exelsa and 'Interdonat' lemon. Madam Vinous, rough lemon and Mexican lime were the best hosts for dsRNA analysis. ELISA values were highest in April (OD405nm = 0.476), decreased steadily until August, and then increased gradually through December. ELISA values were lowest in July and August (OD405nm = 0.157 and 0.141, respectively). dsRNA recovery from a field tree infected with isolate Igdir D was good in March, April and May and poor in January and February. No dsRNA band was detected in August or September.
dc.identifier.doi10.1007/BF02979690
dc.identifier.endpage428
dc.identifier.issn0334-2123
dc.identifier.issue4
dc.identifier.scopus2-s2.0-0036038119
dc.identifier.scopusqualityQ2
dc.identifier.startpage420
dc.identifier.urihttps://doi.org/10.1007/BF02979690
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21065
dc.identifier.volume30
dc.identifier.wosWOS:000177202400011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPriel Publ
dc.relation.ispartofPhytoparasitica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectdouble-stranded RNA
dc.subjectextraction
dc.subjectelectrophoresis
dc.subjectantigen concentration
dc.titleInvestigation of biological properties, double-stranded RNA patterns and antigen concentration in citrus species infected with citrus tristeza virus
dc.typeArticle

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