A new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation

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dc.authoridARMAN, KAIFEE/0000-0002-4667-4655
dc.contributor.authorSakalar, Ergun
dc.contributor.authorArman, Kaifee
dc.date.accessioned2025-01-27T20:26:51Z
dc.date.available2025-01-27T20:26:51Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRandom amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to evaluate the effects of irradiation on the DNA of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata). Meats of sea bass and sea bream were exposed to 0.25, 0.5, 1, 3, 5, and 7 kGy of irradiation (dosage rate 1.31 kGy/h) in a gamma cell at room temperature of approximately 10A degrees C. RAPD-PCR was carried out using DNA samples of both control and irradiated groups. Gel electrophoresis showed loss of some amplicons, especially in the upper long bands of irradiated samples. Due to DNA damage caused by irradiation, the visibility of bands decreased as the radiation dosage increased. This study can be used as a basis for screening of irradiated samples based on loss of specific bands for a specific amount of irradiation.
dc.description.sponsorshipFood Gene Analytic DNA Technology Design (Gidagen), Istanbul, Turkey
dc.description.sponsorshipThis research was supported by Food Gene Analytic DNA Technology Design (Gidagen), Istanbul, Turkey.
dc.identifier.doi10.1007/s10068-015-0158-0
dc.identifier.endpage1237
dc.identifier.issn1226-7708
dc.identifier.issn2092-6456
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84938356755
dc.identifier.scopusqualityQ2
dc.identifier.startpage1233
dc.identifier.urihttps://doi.org/10.1007/s10068-015-0158-0
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22463
dc.identifier.volume24
dc.identifier.wosWOS:000358801400007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherKorean Society Food Science & Technology-Kosfost
dc.relation.ispartofFood Science and Biotechnology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectRAPD-PCR
dc.subjectirradiation
dc.subjectfish
dc.subjectDNA
dc.subjectdamage
dc.titleA new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation
dc.typeArticle

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