Photoelectrochemical glucose biosensor in flow injection analysis system based on glucose dehydrogenase immobilized on poly-hematoxylin modified glassy carbon electrode

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dc.authoridGiray Dilgin, Didem/0000-0002-3487-3470
dc.contributor.authorDilgin, Didem Giray
dc.contributor.authorGokcel, H. Ismet
dc.date.accessioned2025-01-27T20:47:29Z
dc.date.available2025-01-27T20:47:29Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, a photoelectrochemical glucose biosensor is presented, comprising electropolymerized hematoxylin film on poly-amidoamine (PAMAM) dendrimers adsorbed on glassy carbon electrode (polyHT/PAMAM/GCE). After immobilization of glucose dehydrogenase (GDH) onto the poly-HT/PAMAM/GCE, photoelectrochemical biosensing of glucose was investigated using cyclic voltammetry and amperometry in a flow injection analysis (FIA) system dependent on the NAD+/NADH redox coupledehydrogenase enzyme. The linear range was from 1 x 10(-5) M to 1 x 10(-3) M with a sensitivity of 0.76 mu A mM(-1) and detection limit of 3.0 mM without irradiation in the FIA system. After irradiation, the linear range was from 5 x 10(-6) M to 1 x 10(-3) M with a sensitivity of 1.90 mu A mM(-1) and a detection limit of 1.5 mM. Comparing the results obtained from irradiation of the electrode surface with those from the reaction without irradiation, the sensitivity and the detection limit increased around 2.5- and 2.0-fold, respectively. The photoelectrochemical biosensor showed good performance with high upper detection limit, acceptable repeatability, and selectivity, providing a rapid alternative method for monitoring biomolecules and extending photoelectrochemical determination in the FIA system. The proposed electrochemical and photoelectrochemical biosensor was successfully applied to determination of glucose in real samples. The results obtained from this study provide a basis for future studies in these directions.
dc.identifier.doi10.1039/c4ay02269f
dc.identifier.endpage999
dc.identifier.issn1759-9660
dc.identifier.issn1759-9679
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84922429700
dc.identifier.scopusqualityQ1
dc.identifier.startpage990
dc.identifier.urihttps://doi.org/10.1039/c4ay02269f
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24926
dc.identifier.volume7
dc.identifier.wosWOS:000349679300023
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofAnalytical Methods
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectNicotinamide Adenine-Dinucleotide
dc.subjectPhotoelectrocatalytic Oxidation
dc.subjectElectrocatalytic Oxidation
dc.subjectAmperometric Biosensors
dc.subjectPaste Electrodes
dc.subjectNadh Oxidation
dc.subjectVisible Irradiation
dc.subjectTransfer Mediator
dc.subjectToluidine Blue
dc.subjectNanotubes
dc.titlePhotoelectrochemical glucose biosensor in flow injection analysis system based on glucose dehydrogenase immobilized on poly-hematoxylin modified glassy carbon electrode
dc.typeArticle

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