Genotoxic effects of chlorophenoxy herbicide diclofop-methyl in mice in vivo and in human lymphocytes in vitro

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dc.authoridYUZBASIOGLU, DENIZ/0000-0003-2756-7712
dc.authoridYILMAZ, SERKAN/0000-0001-8641-9475
dc.authoridUNAL, FATMA/0000-0002-7468-6186
dc.contributor.authorUnal, Fatma
dc.contributor.authorYuzbasioglu, Deniz
dc.contributor.authorYilmaz, Serkan
dc.contributor.authorAkinci, Nihan
dc.contributor.authorAksoy, Huseyin
dc.date.accessioned2025-01-27T20:50:19Z
dc.date.available2025-01-27T20:50:19Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractDiclofop-methyl (DM) is a chlorophenoxy derivative used in large quantities for the control of annual grasses in grain and vegetable crops. In this study, the genotoxic effects of DM were investigated by measuring chromosomal aberrations (CAs) in mouse bone-marrow cells and CA and the comet assay in human peripheral lymphocytes. Mice were treated with 15.63, 31.25, 62.5, and 125 mg/kg body weight of DM intraperitoneally for 24 hours, and 15.63-, 31.25-, 62.5-, 125-, and 250-mu g/mL concentrations were applied to human lymphocytes for both 24 and 48 hours. In in vivo treatments, DM significantly, but not dose dependently, increased the total chromosome aberrations, compared to both negative and solvent controls. Cell proliferation was significantly, but not dose dependently, affected by all doses. In in vitro treatments, DM (except 15.63 mu g/mL) significantly and dose dependently increased the frequency of chromosome aberrations. Also, 250 mu g/mL of 48-hour treatment was found to be toxic. Cell proliferation was significantly and dose dependently affected by DM applications, when compared to negative control. In in vitro treatments, DM significantly decreased the mitotic index only at the highest concentration for 24 hours, and 62.5- and 125-mu g/mL concentrations for 48 hours. In the comet assay, a significant and dose-dependent increase in comet-tail intensity was observed at 62.5-, 125-, and 250-mu g/mL concentrations. The mean comet-tail length was significantly increased in all concentrations. Our results demonstrate that DM is genotoxic in mammalian cells in vivo and in vitro.
dc.description.sponsorshipGazi University [05/2006-23]
dc.description.sponsorshipThis study was supported by a Gazi University research fund (project no.: 05/2006-23).
dc.identifier.doi10.3109/01480545.2010.538695
dc.identifier.endpage395
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue4
dc.identifier.pmid21714768
dc.identifier.scopus2-s2.0-80052732257
dc.identifier.scopusqualityQ1
dc.identifier.startpage390
dc.identifier.urihttps://doi.org/10.3109/01480545.2010.538695
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25468
dc.identifier.volume34
dc.identifier.wosWOS:000294861800007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDiclofop-methyl
dc.subjectgenotoxicity
dc.subjectmouse bone-marrow cells
dc.subjecthuman lymphocytes
dc.subjectchromosomal aberrations
dc.subjectcomet assay
dc.titleGenotoxic effects of chlorophenoxy herbicide diclofop-methyl in mice in vivo and in human lymphocytes in vitro
dc.typeArticle

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