Molecular DNA-based detection of ionising radiation in meat

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dc.contributor.authorSakalar, Ergun
dc.date.accessioned2025-01-27T20:54:19Z
dc.date.available2025-01-27T20:54:19Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBACKGROUNDIonising radiation induces molecular alterations, such as formation of ions, free radicals, and new stable molecules, and cleavage of the chemical bonds of the molecules present in food. Irradiation-treated meat should be labelled to control the process and to ensure free consumer choice. Therefore, sensitive analytical methods are required to detect the irradiation dose. RESULTSMeat samples were exposed to radiation doses of 0, 0.272, 0.497, 1.063, 3.64, 8.82 and 17.42 kGy in an industrial Co-60 gamma cell. Primers were designed to amplify 998, 498 and 250-base pair (bp) regions of the 18S rRNA gene of nuclear DNA from the irradiated samples. A new DNA-based method was developed to quantify the radiation exposed to the unstored meat and the meat stored at -20 degrees C for 3 and 6 months. The method was able to detect meat samples stored and unstored with dose limits of 1.063 and 3.64 kGy, respectively. CONCLUSIONThe level of irradiation can be detected using primer pairs that target particularly different-sized sequences for DNA amplification by PCR. This method can be widely used for the analysis of not only meat samples, but also all biological materials containing DNA. (c) 2016 Society of Chemical Industry
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113Z831]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with Project Grant No. 113Z831.
dc.identifier.doi10.1002/jsfa.8015
dc.identifier.endpage2106
dc.identifier.issn0022-5142
dc.identifier.issn1097-0010
dc.identifier.issue7
dc.identifier.pmid27570232
dc.identifier.scopus2-s2.0-84991716620
dc.identifier.scopusqualityQ1
dc.identifier.startpage2100
dc.identifier.urihttps://doi.org/10.1002/jsfa.8015
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26045
dc.identifier.volume97
dc.identifier.wosWOS:000398686200015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of The Science of Food and Agriculture
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectionising radiation
dc.subjectreal-time PCR
dc.subjectDNA damage
dc.subjectdose detection
dc.subjectcomet assay
dc.titleMolecular DNA-based detection of ionising radiation in meat
dc.typeArticle

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