Ameliorative potential of Lavandula stoechas in metabolic syndrome via multitarget interactions

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dc.authoridSezerman, Osman Ugur/0000-0003-0905-6783
dc.authoridEKREN, Ruchan/0000-0001-6737-7281
dc.authoridTumer, Tugba/0000-0002-1740-4867
dc.contributor.authorKulabas, S. S.
dc.contributor.authorIpek, H.
dc.contributor.authorTufekci, A. R.
dc.contributor.authorArslan, S.
dc.contributor.authorDemirtas, I.
dc.contributor.authorEkren, R.
dc.contributor.authorSezerman, U.
dc.date.accessioned2025-01-27T20:39:07Z
dc.date.available2025-01-27T20:39:07Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractEthnopharmacological importance: Decoction and infusion prepared from aerial parts of Lavandula stoechas L. (L. stoechas) have been traditionally used as remedy against several components of metabolic syndrome (MetS) and associated disorders including type II diabetes and cardiovascular diseases by Anatolian people. Aim of the study: The aim is to elucidate the potential ameliorative effects of L. stoechas aqueous extracts on insulin resistance and inflammation models through multitarget in vitro approaches and also to elucidate mechanism of action by analyzing transcriptional and metabolic responses. Materials and methods: An aqueous extract was prepared and fractionated to give rise to ethyl acetate (EE) and butanol (BE) extracts. The anti-insulin resistance effects of BE and EE were evaluated on palmitate induced insulin resistance model of H4IIE, C2C12 and 3T3L1 cells by using several metabolic parameters. Specifically, whole genome transcriptome analysis was performed by using microarray over 55.000 genes in control, insulin resistant and EE (25 mu g/mL) treated insulin resistant H4IIE cells. Anti-inflammatory effects of both extracts were analyzed in LPS-stimulated RAW264.7 macrophages. Results: Both EE and BE at low doses (25-50 mu g/mL) significantly decreased hepatic gluconeogenesis in H4IIE cell line by suppressing the expression of PEPCK and G6Pase. In C2C12 myotubes, both extracts increased the insulin stimulated glucose uptake more effectively than metformin. Both extracts decreased the isoproterenol induced lipolysis in 3T3L1 cell line. Moreover, they also effectively increased the expression of lipoprotein lipase protein level in insulin resistant myotubes at low doses. EE increased the protein level of PPARy and stimulated the activation AKT in insulin resistant H4IIE and C2C12 cell lines. The results obtained from biochemical assays, mRNA/protein studies and whole genome transcriptome analyses were found to be complementary and provided support for the hypothesis that EE might be biologically active against insulin resistance and act through the inhibition of liver gluconeogenesis and AKT activation. Besides, LPS induced inflammation in RAW264.7 macrophages was mainly inhibited by EE through suppression of iNOS/NO signaling, IMO and COX-2 genes. HPLC-TOF/MS analysis of EE of L. stoechas mainly resulted in caffeic acid, apigenin, luteolin, rosmarinic acid and its methyl ester, 4-hydroxybenzoic acid, vanillic acid, ferrulic acid and salicylic acid. Conclusion: Data suggest that EE of L. stoechas contains phytochemicals that can be effective in the treatment/ prevention of insulin resistance and inflammation. These results validate the traditional use of L. stoechas in Anatolia against several metabolic disorders including metabolic syndrome.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112T442]; Canakkale Onsekiz Mart University [FYL-2015-434, FDK-2017-1132]
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK Project ID: 112T442) supported this study. Bioactivity studies related with the anti-inflammatory part were partially supported by Canakkale Onsekiz Mart University, Scientific Research Projects (FYL-2015-434 and FDK-2017-1132).
dc.identifier.doi10.1016/j.jep.2018.04.043
dc.identifier.endpage98
dc.identifier.issn0378-8741
dc.identifier.pmid29729383
dc.identifier.scopus2-s2.0-85047413423
dc.identifier.scopusqualityQ1
dc.identifier.startpage88
dc.identifier.urihttps://doi.org/10.1016/j.jep.2018.04.043
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23866
dc.identifier.volume223
dc.identifier.wosWOS:000437383400009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofJournal of Ethnopharmacology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectLavandula stoechas
dc.subjectInsulin resistance
dc.subjectGluconeogenesis
dc.subjectGlucose uptake
dc.subjectLipolysis
dc.subjectTranscriptome
dc.titleAmeliorative potential of Lavandula stoechas in metabolic syndrome via multitarget interactions
dc.typeArticle

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